Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative
Gao, M; Zhang, X; Wang, Y; Liu, QL; Yu, FB; Huang, Y; Ding, CF; Chen, LX
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
2019-06-18
Volume91Issue:12Pages:7774-7781
KeywordREACTIVE OXYGEN SULFANE SULFUR LIVING CELLS OXIDATIVE STRESS CHEMICAL PROBES ON PROBE SULFIDE NITROREDUCTASE LUMINESCENT PEROXYNITRITE
MOST Discipline CatalogueChemistry, Analytical
DOI10.1021/acs.analchem.9b01189
Contribution Rank[Gao, Min; Zhang, Xia; Wang, Yue; Huang, Yan; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; [Liu, Qingluan] China Med Univ, Div Clin Med 3, Shenyang 110122, Liaoning, Peoples R China; [Ding, Caifeng] Qingdao Univ Sci & Technol, MOE, Key Lab Opt Electr Sensing & Analyt Chem Life Sci, Qingdao 266042, Shandong, Peoples R China; [Ding, Caifeng] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Shandong, Peoples R China; [Gao, Min; Zhang, Xia; Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
Department海岸带环境工程技术研究与发展中心
AbstractChronic hypoxic stress disrupts the intracellular redox homeostasis, leads to a series of physiological dysfunction, and finally results in many diseases including cancer and inflammatory and cardiovascular diseases. The intracellular redox status is related to the homeostasis between reactive oxygen species (ROS) and cellular antioxidant species. Superoxide anion (O-2(center dot-) is considered to be a precursor of ROS. As a member of reactive sulfur species, hydrogen polysulfides (H2Sn) are a class of antioxidants in cells, which act as an important regulator for the intracellular redox state. Therefore, trapping the cross-talk of O-2(center dot-) and H2Sn is a benefit for further understanding the physiological and pathological effects. Herein, we conceive a fluorescent probe HCy-ONO for sequential detection of O-2(center dot-) and H2Sn in cells and in mouse models. Based on a tandem reaction, the probe HCy-ONO can be used to detect O-2(center dot-) and H2Sn in different fluorescence collection windows without spectral overlap interference with limits of detection 90 and 100 nM, respectively. The strategy affords high sensitivity and selectivity for our detection in living cell models under continuous hypoxic and intermittent hypoxic conditions, revealing the reason for ischemia-reperfusion injury. Moreover, the probe can distinguish the inflamed tissue from normal tissue in acute peritonitis mouse model. Finally, our probe is successfully applied for imaging of O-2(center dot-) and H2Sn in the SH-SYSY tumor-bearing mouse model, which is helpful to elucidate the physiological and pathological processes. These data demonstrated that different hypoxic status lead to different concentrations between H2Sn and O-2(center dot-).
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordREACTIVE OXYGEN ; SULFANE SULFUR ; LIVING CELLS ; OXIDATIVE STRESS ; CHEMICAL PROBES ; ON PROBE ; SULFIDE ; NITROREDUCTASE ; LUMINESCENT ; PEROXYNITRITE
WOS Research AreaChemistry, Analytical
WOS IDWOS:000472682000037
Funding OrganizationNational Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21775162, 41776110] ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CASChinese Academy of Sciences [KF2016-22] ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24918
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China;
3.China Med Univ, Div Clin Med 3, Shenyang 110122, Liaoning, Peoples R China;
4.Qingdao Univ Sci & Technol, MOE, Key Lab Opt Electr Sensing & Analyt Chem Life Sci, Qingdao 266042, Shandong, Peoples R China;
5.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Shandong, Peoples R China;
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
7.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
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Gao, M,Zhang, X,Wang, Y,et al. Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative[J]. ANALYTICAL CHEMISTRY,2019,91(12):7774-7781.
APA Gao, M.,Zhang, X.,Wang, Y.,Liu, QL.,Yu, FB.,...&Chen, LX.(2019).Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative.ANALYTICAL CHEMISTRY,91(12),7774-7781.
MLA Gao, M,et al."Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative".ANALYTICAL CHEMISTRY 91.12(2019):7774-7781.
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