A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode

doi:10.1016/j.fsi.2019.07.031

	A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode
	Yang, DL; Han, YJ ; Chen, LZ ; Cao, RW ; Wang, Q; Dong, ZJ ; Liu, H; Zhang, XL; Zhang, QQ; Zhao, JM
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2019-09
卷号	92 页码:897-904
关键词	Venerupis philippinarum Macin Molecular characterization Antimicrobial activity
研究领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2019.07.031
产权排序	[Yang, Dinglong; Han, Yijing; Cao, Ruiwen; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
通讯作者	Zhao, Jianmin(jmzhao@yic.ac.cn)
作者部门	中国科学院牟平海岸带环境综合试验站
英文摘要	In the present study, a macin was cloned and characterized from clam Venerupis philippinarum (designed as VpMacin). The full-length cDNA of VpMacin was of 579 bp, encoding a peptide of 87 amino acids with the predicted molecular weight of 9.7 kDa. Analysis of the conserved domain suggested that VpMacin was a new member of the macin family. In non-stimulated clams, VpMacin transcripts exhibited different tissue expression pattem, and highly expressed in the tissues of gills and hepatopancreas. Generally, the temporal expression of VpMacin transcripts was significantly induced in hemocytes of clams post Vibrio anguillarum challenge. Moreover, the recombinant VpMacin protein (rVpMacin) showed obvious antimicrobial activities against Grampositive and Gram-negative bacteria. After incubated with 40 mu M rVpMacin, all detected Escherichia coli could be killed within 60 min. Membrane integrity analysis revealed that rVpMacin could increase the membrane permeability of bacteria and then resulted in cell death. Overall, our results suggested that VpMacin had an important function in host defense against invasive pathogens.
文章类型	Article
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41476126, 41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; Youth Innovation Promotion Association of CAS [2016196]
收录类别	SCI
语种	英语
关键词[WOS]	CLAM RUDITAPES-PHILIPPINARUM ; CATIONIC ANTIMICROBIAL PEPTIDES ; MOLECULAR CHARACTERIZATION ; INNATE IMMUNITY ; VIBRIO-TAPETIS ; DEFENSIN ; MECHANISMS ; EXPRESSION ; FAMILY ; INVOLVEMENT
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000483634300097
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24873
专题	支撑部门_中国科学院牟平海岸带环境综合试验站海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China; 4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Yang, DL,Han, YJ,Chen, LZ,et al. A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode[J]. FISH & SHELLFISH IMMUNOLOGY,2019,92:897-904.
APA	Yang, DL.,Han, YJ.,Chen, LZ.,Cao, RW.,Wang, Q.,...&Zhao, JM.(2019).A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode.FISH & SHELLFISH IMMUNOLOGY,92,897-904.
MLA	Yang, DL,et al."A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode".FISH & SHELLFISH IMMUNOLOGY 92(2019):897-904.

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