Peak selection matters in principal component analysis: A case study of syntrophic microbes | |
Yang, CY; Wei, WC; Liu, FH; Yu, XY | |
发表期刊 | BIOINTERPHASES |
ISSN | 1934-8630 |
2019-09 | |
卷号 | 14期号:5页码:51004 |
关键词 | ION MASS-SPECTROMETRY INTERSPECIES ELECTRON-TRANSFER GEOBACTER-SULFURREDUCENS SP-NOV TIME REDUCTION BIOFILMS SIMS SURFACES ADHESION |
研究领域 | Biophysics ; Materials Science, Biomaterials |
DOI | 10.1116/1.5118237 |
产权排序 | [Yang, Cuiyun; Wei, Wenchao; Liu, Fanghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China; [Yang, Cuiyun; Yu, Xiao-Ying] Pacific Northwest Natl Lab, Energy & Environm Directorate, Richland, WA 99354 USA |
通讯作者 | Liu, Fanghua(fhliu@yic.ac.cn) |
作者部门 | 海岸带生物学与生物资源保护实验室 |
英文摘要 | In situ liquid time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful technique to study surface characterization of living biofilms in hydrated conditions. However, ToF-SIMS data analysis is still a great challenge in complicated bacterial biofilms, because many interference peaks from the medium may result in inaccurate interpretation. In this study, two syntrophic Geobacter populations are investigated using in situ liquid ToF-SIMS to reveal the biofilm surface changes between them due to direct interspecies electron transfer. By comparing spectral principal component analysis (PCA) results of all peaks and selected peaks, the authors find that spectral peak overlay is an effective strategy to reduce the matrix effect in handling complex ToF-SIMS data. Additionally, the spectral PCA results of high intensity and high resolution data obtained from liquid ToF-SIMS are compared. Selected peaks, amino acid peaks, and water cluster peaks spectral PCA produce nice separation among samples in both high intensity and high resolution data sets. However, the high resolution data show better separation between coculture planktonic and coculture aggregates, confirming that the higher mass accuracy is useful in the analysis of microbial samples. In conclusion, the results show that peak selection is critical for acquiring effective microbial information and interpretation of syntrophic Geobacter using spectral data from in situ liquid ToF-SIMS. |
文章类型 | Article |
资助机构 | Pacific Northwest National Laboratory (PNNL) ; Earth and Biological Sciences Directorate (EBSD) mission seed Laboratory Directed Research and Development (LDRD) ; China National Scholarship ; Office of Biological and Environmental Research [49694, 50569] ; PNNL Alternate Sponsored Fellowship |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | ION MASS-SPECTROMETRY ; INTERSPECIES ELECTRON-TRANSFER ; GEOBACTER-SULFURREDUCENS ; SP-NOV ; TIME ; REDUCTION ; BIOFILMS ; SIMS ; SURFACES ; ADHESION |
研究领域[WOS] | Biophysics ; Materials Science, Biomaterials |
WOS记录号 | WOS:000487798900001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24865 |
专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China; 2.Pacific Northwest Natl Lab, Energy & Environm Directorate, Richland, WA 99354 USA |
推荐引用方式 GB/T 7714 | Yang, CY,Wei, WC,Liu, FH,et al. Peak selection matters in principal component analysis: A case study of syntrophic microbes[J]. BIOINTERPHASES,2019,14(5):51004. |
APA | Yang, CY,Wei, WC,Liu, FH,&Yu, XY.(2019).Peak selection matters in principal component analysis: A case study of syntrophic microbes.BIOINTERPHASES,14(5),51004. |
MLA | Yang, CY,et al."Peak selection matters in principal component analysis: A case study of syntrophic microbes".BIOINTERPHASES 14.5(2019):51004. |
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