A bactericidal permeability-increasing protein (BPI) from manila clam Ruditapes philippinarum: Investigation on the antibacterial activities and antibacterial action mode | |
Yang, DL; Han, YJ; Chen, LZ; Cao, RW; Wang, Q; Dong, ZJ; Liu, H; Zhang, XL; Zhang, QQ; Zhao, JM | |
发表期刊 | FISH & SHELLFISH IMMUNOLOGY |
ISSN | 1050-4648 |
2019-10 | |
卷号 | 93页码:841-850 |
关键词 | Ruditapes philippinarum Bactericidal permeability-increasing protein Antimicrobial activity Membrane disruption |
研究领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
DOI | 10.1016/j.fsi.2019.08.050 |
产权排序 | [Yang, Dinglong; Han, Yijing; Cao, Ruiwen; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China; [Han, Yijing; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China |
通讯作者 | Zhao, Jianmin(jmzhao@yic.ac.cn) |
作者部门 | 中国科学院牟平海岸带环境综合试验站 |
英文摘要 | Bactericidal permeability-increasing protein (BPI) is an antimicrobial protein with potent endotoxin-neutralising activity and plays a crucial role in innate immunity against bacterial infection. In the present study, a bpi (designed as rpbpi) was identified and characterized from manila clam Ruditapes philippinarion. Multiple alignments and phylogenetic analysis suggested that rpbpi was a new member of the bpis family. In non-stimulated clams, rpbpi transcripts were ubiquitously expressed in all tested tissues with the highest expression level in hemocytes. After Vibrio anguillarum challenge, the expression levels of rpbpi mRNA in hemocytes were up-regulated significantly at 3 h and 48 h compared with that in the control, which were 4.01- and 19.10-fold (P < 0.05), respectively. The recombinant RpBPI (rRpBPI) showed high antibacterial activities against Gram-negative bacteria Escherichia coli and V. anguillarum, but not Staphylococcus aureus. Moreover, membrane integrity analysis revealed that rRpBPI increased the membrane permeability of Gram-negative bacteria, and then resulted in cell death. Overall, our results suggested that RpBPI played an important role in the elimination of invaded bacteria through membrane-disruptive activity. |
文章类型 | Article |
资助机构 | Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303, KZZD-EW-14, KFJ-STS-ZDTP-023] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196] ; Two-Hundred Talents Plan of Yantai [Y839081021] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; Youth Innovation Promotion Association, CAS [2016196] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | LIPOPOLYSACCHARIDE-BINDING PROTEIN ; GRAM-NEGATIVE BACTERIA ; ANTIMICROBIAL PEPTIDES ; IDENTIFICATION ; EXPRESSION ; MECHANISMS ; HEMOCYTES ; FRAGMENT ; DEFENSE ; DISEASE |
研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS记录号 | WOS:000488998300093 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24847 |
专题 | 支撑部门_中国科学院牟平海岸带环境综合试验站 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 2.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, DL,Han, YJ,Chen, LZ,et al. A bactericidal permeability-increasing protein (BPI) from manila clam Ruditapes philippinarum: Investigation on the antibacterial activities and antibacterial action mode[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:841-850. |
APA | Yang, DL.,Han, YJ.,Chen, LZ.,Cao, RW.,Wang, Q.,...&Zhao, JM.(2019).A bactericidal permeability-increasing protein (BPI) from manila clam Ruditapes philippinarum: Investigation on the antibacterial activities and antibacterial action mode.FISH & SHELLFISH IMMUNOLOGY,93,841-850. |
MLA | Yang, DL,et al."A bactericidal permeability-increasing protein (BPI) from manila clam Ruditapes philippinarum: Investigation on the antibacterial activities and antibacterial action mode".FISH & SHELLFISH IMMUNOLOGY 93(2019):841-850. |
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