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A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo
Wang, Y; Gao, M; Liao, CY; Yu, FB; Chen, LX
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
2019-12-12
卷号301页码:127038
关键词Mercury poisoning Near-infrared fluorescent probe Ratiometric biomaging Superoxide anion Mercury ion
研究领域Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
DOI10.1016/j.snb.2019.127038
产权排序[Wang, Yue; Gao, Min; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liao, Chunyang] Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China; [Wang, Yue; Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Yu, Fabiao(fbyu@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Mercury exposure is associated with severe oxidative stress especially superoxide anion radicals (O-2(center dot-)). Mercury exposure is common in the clinic, but it is difficult to track. Although many researchers have tried to reveal the mechanism of mercury exposure, it is still remain vague that the differences between acute mercury exposure and chronic mercury exposure as well as the fluctuations in O-2(center dot-) during mercury ion (Hg2+) stress. Thus effective tool for O-2(center dot-) and Hg2+ associated-detection is needed urgently. Herein we have developed a stable near-infrared ratiometric fluorescent probe, HCy-SH, for O-2(center dot-) and Hg2+ associated-detection. Probe HCy-SH was designed and synthesized based on a heptamethine cyanine fluorophore and a thiol-responsive group. The probe HCy-SH can be used for mercury poisoning detection in HEK 293 cells and mice models with low detection limits of 65 nM for O-2(center dot-) and 72 nM for Hg2+. Relying on the probe HCy-SH, we found that O-2(center dot-) burst was much severer in acute mercury exposure than chronic mercury exposure, especially in heart, and Hg2+ mainly accumulated in kidney no matter acute mercury exposure or chronic mercury exposure. The experimental results indicated that the probe HCy-SH was a potential candidate for accurate diagnosis and efficacy evaluation of mercury exposure.
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21976209, 21575159, 21775162, 21405172, 21522706] ; program of Youth Innovation Promotion Association, CAS [2015170] ; Research Center for Eco-Environmental Sciences, CASChinese Academy of Sciences [KF2016-22]
收录类别SCI
语种英语
关键词[WOS]ENDOGENOUS SUPEROXIDE ; VISUAL DETECTION ; HG2+ ; STRESS ; FRET
研究领域[WOS]Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000489529300018
引用统计
被引频次：37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/24792
专题中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
中国科学院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Y,Gao, M,Liao, CY,et al. A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,301:127038.
APA Wang, Y,Gao, M,Liao, CY,Yu, FB,&Chen, LX.(2019).A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo.SENSORS AND ACTUATORS B-CHEMICAL,301,127038.
MLA Wang, Y,et al."A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo".SENSORS AND ACTUATORS B-CHEMICAL 301(2019):127038.
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