Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays | |
Wu, Y; Choi, N; Chen, H; Dang, H; Chen, LX![]() | |
发表期刊 | ANALYTICAL CHEMISTRY
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ISSN | 0003-2700 |
2020-02-04 | |
卷号 | 92期号:3页码:2628-2634 |
关键词 | MULTIPLEX DETECTION AMPLIFICATION-FREE SERS PCR DNA SPECTROSCOPY IMMUNOASSAY MUTATIONS DIAGNOSIS |
研究领域 | Chemistry, Analytical |
DOI | 10.1021/acs.analchem.9b04522 |
产权排序 | [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wu, Yixuan; Chen, Hao; Dang, Hajun; Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; [Choi, Namhyun] Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea |
通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr) |
作者部门 | 海岸带环境工程技术研究与发展中心 |
英文摘要 | We report a surface-enhanced Raman scattering (SERS)-based polymerase chain reaction (PCR) assay platform for the sensitive and rapid detection of a DNA marker (pagA) of Bacillus anthracis. Real-time quantitative PCR (RT-qPCR) has been recently considered a gold standard for the quantitative evaluation of a target gene, but it still suffers from the problem of a long thermocycling time. To address this issue, we developed a conceptually new SERS-PCR platform and evaluated its performance by sequentially measuring the Raman signals of B. anthracis DNA after the completion of different thermocycling numbers. According to our experimental data, SERS-PCR has lower limits of detection (LODs) than RT-qPCR under the small cycle number of 20. Particularly, it was impossible to detect a target DNA amplicon using RT-qPCR before the number of cycles reached 15, but SERS-PCR enabled DNA detection after only five cycles with an LOD value of 960 pM. In addition, the dynamic range for SERS-PCR (0.1-1000 pM) is wider than that for RTqPCR (150-1000 pM) under the same condition. We believe that this SERS-PCR technique has a strong potential to be a powerful tool for the rapid and sensitive diagnosis of infectious diseases in the near future. |
文章类型 | Article |
资助机构 | National Research Foundation of KoreaNational Research Foundation of Korea [2019R1A2C3004375] ; Chung-Ang University |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | MULTIPLEX DETECTION ; AMPLIFICATION-FREE ; SERS ; PCR ; DNA ; SPECTROSCOPY ; IMMUNOASSAY ; MUTATIONS ; DIAGNOSIS |
研究领域[WOS] | Chemistry, Analytical |
WOS记录号 | WOS:000511509400038 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24755 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; 3.Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea |
推荐引用方式 GB/T 7714 | Wu, Y,Choi, N,Chen, H,et al. Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays[J]. ANALYTICAL CHEMISTRY,2020,92(3):2628-2634. |
APA | Wu, Y,Choi, N,Chen, H,Dang, H,Chen, LX,&Choo, J.(2020).Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays.ANALYTICAL CHEMISTRY,92(3),2628-2634. |
MLA | Wu, Y,et al."Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays".ANALYTICAL CHEMISTRY 92.3(2020):2628-2634. |
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