A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model

doi:10.1039/c9an02436k

YIC-IR > 中国科学院海岸带环境过程与生态修复重点实验室 > 海岸带环境工程技术研究与发展中心

	A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model
	Wang, Y; Han, XY ; Zhang, X ; Zhang, L; Chen, LX
发表期刊	ANALYST
ISSN	0003-2654
	2020-02-21
卷号	145 期号:4 页码:1389-1395
关键词	NITROREDUCTASE DETECTION RESISTANCE
研究领域	Chemistry, Analytical
DOI	10.1039/c9an02436k
产权排序	[Wang, Yue; Han, Xiaoyue; Zhang, Xia; Zhang, Li; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Yue; Zhang, Xia; Zhang, Li] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
通讯作者	Chen, Lingxin(lxchen@yic.ac.cn)
作者部门	海岸带环境工程技术研究与发展中心
英文摘要	Nitroreductase (NTR) with a high expression level in tumors has been considered as a biomarker of highly aggressive hypoxia tumors. Thus, it is important to develop powerful tools for tumor hypoxia detection. Here, we developed a two-photon fluorescent probe hTP-NNO2 for NTR detection. The probe with one-step synthesis exhibited high yield. hTP-NNO2 showed high selectivity and sensitivity for NTR and the detection limit was as low as 43 ng mL(-1). hTP-NNO2 also showed low cytotoxicity and high stability, indicating that hTP-NNO2 is suitable for NTR detection in real-time and in situ under physiological conditions. hTP-NNO2 was used for NTR imaging in hypoxia cells and the fluorescence intensity of hTP-NNO2 increased with decreasing oxygen concentration. Benefiting from the advantages of two-photon fluorescent probes, we performed NTR detection in deep brain tissue with an imaging depth of up to 100 mu m. hTP-NNO2 was further successfully applied for NTR detection in zebrafish and tumors. These results indicated that we developed a promising fluorescence imaging tool for NTR detection in vitro and in vivo.
文章类型	Article
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21976209, 21575159, 21778026] ; Taishan Scholar Project
收录类别	SCI
语种	英语
关键词[WOS]	NITROREDUCTASE DETECTION ; RESISTANCE
研究领域[WOS]	Chemistry, Analytical
WOS记录号	WOS:000515832700030
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24748
专题	中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室中国科学院烟台海岸带研究所知识产出中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Y,Han, XY,Zhang, X,et al. A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model[J]. ANALYST,2020,145(4):1389-1395.
APA	Wang, Y,Han, XY,Zhang, X,Zhang, L,&Chen, LX.(2020).A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model.ANALYST,145(4),1389-1395.
MLA	Wang, Y,et al."A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model".ANALYST 145.4(2020):1389-1395.

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