| A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT | |
Han, Xiaoyue; Song, Xinyu; Li, Bowei ; Yu, Fabiao; Chen, Lingxin
| |
| 发表期刊 | BIOMATERIALS SCIENCE
 |
| ISSN | 2047-4830 |
| 2018-03-01 | |
| 卷号 | 6期号:3页码:672-682 |
| 关键词 | TURN-ON PROBE HYDROGEN-SULFIDE IN-VIVO CHEMICAL BIOLOGY OXIDATIVE STRESS RATIONAL DESIGN SYNTHASE CBS FAR-RED POLYSULFIDES CHEMISTRY |
| 研究领域 | Materials Science, Biomaterials |
| DOI | 10.1039/c7bm00951h |
| 产权排序 | [Han, Xiaoyue; Song, Xinyu; Li, Bowei; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Fabiao; Chen, Lingxin] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China |
| 作者部门 | 海岸带环境工程技术研究与发展中心 |
| 英文摘要 | Sulfane sulfur refers to ionized sulfur that is reversibly attached to other sulfur atoms in the form of 6-valence electrons (S-0). Sulfane sulfur possesses stronger nucleophilicity and reducibility than hydrogen sulfide in a series of physiological reactions, which probably makes sulfane sulfur the actual signal molecule in cells. Herein, we designed and synthesized a near-infrared (NIR) fluorescent probe BD-diSH for sensitive detection and imaging of sulfane sulfur in living cells and in vivo. The probe BD-diSH is composed of two moieties: the fluorophore azo-BODIPY and the sulfane sulfur recognition unit, viz., 2-mercapto benzoate. BD-diSH displayed high sensitivity and selectivity towards sulfane sulfur. The mercapto group (-SH) of 2-mercapto benzoic acid can nucleophilically capture the sulfur atom of thiosulfoxide tautomers in sulfane sulfur to form -SSH. The group -SSH will immediately induce intramolecular cyclization reaction and release the azo-BODIPY fluorophore to emit NIR fluorescence. The probe BD-diSH was successfully applied to detect and image sulfane sulfur in the cytoplasm of the living cells. The results illustrated that the endogenous and exogenous sulfane sulfur level changed depending on different cell lines. BD-diSH was also capable of imaging the level changes of sulfane sulfur in mice. The above applications make our new probe a potential chemical tool for the study of physiological and pathological functions of sulfur sulfide in living cells and in vivo. |
| 文章类型 | Article |
| 资助机构 | State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22] ; National Nature Science Foundation of China [21775162, 21405172, 21575159] ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705] ; program of Youth Innovation Promotion Association, CAS [2015170] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | TURN-ON PROBE ; HYDROGEN-SULFIDE ; IN-VIVO ; CHEMICAL BIOLOGY ; OXIDATIVE STRESS ; RATIONAL DESIGN ; SYNTHASE CBS ; FAR-RED ; POLYSULFIDES ; CHEMISTRY |
| 研究领域[WOS] | Materials Science |
| WOS记录号 | WOS:000426392200021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24385 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Xiaoyue,Song, Xinyu,Li, Bowei,et al. A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT[J]. BIOMATERIALS SCIENCE,2018,6(3):672-682. |
| APA | Han, Xiaoyue,Song, Xinyu,Li, Bowei,Yu, Fabiao,&Chen, Lingxin.(2018).A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT.BIOMATERIALS SCIENCE,6(3),672-682. |
| MLA | Han, Xiaoyue,et al."A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT".BIOMATERIALS SCIENCE 6.3(2018):672-682. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A near-infrared fluo（5036KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论