Advanced  
YIC-IR  > 海岸带生物学与生物资源利用所重点实验室  > 期刊论文
题名:
Characterization of syntrophic Geobacter communities using ToF-SIMS
作者: Wei, Wenchao1,2,3; Zhang, Yanyan4; Komorek, Rachel5; Plymale, Andrew6; Yu, Rujia5; Wang, Bingchen1; Zhu, Zihua4; Liu, Fanghua1; Yu, Xiao-Ying2
刊名: BIOINTERPHASES
ISSN号: 1934-8630
出版日期: 2017-12-01
卷号: 12, 期号:5
DOI: 10.1116/1.4986832
产权排序: 第1完成单位
通讯作者: Liu, Fanghua(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China) ; Yu, Xiao-Ying(Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Richland, WA 99354 USA)
英文摘要: The aggregation of syntrophic Geobacter metallireducens and Geobacter sulfurreducens is beneficial for enhancing direct interspecies electron transfer (DIET). Although DIET was suspected to occur on the microbial community surface, the surface chemical speciation of such cocultured communities remains unclear. In order to better understand surface interactions related to DIET, the authors characterized a series of samples associated with syntrophic G. metallireducens and G. sulfurreducens using surface sensitive time-of-flight secondary ion mass spectrometry (ToF-SIMS). Principal component analysis was used in spectral analysis. Our results show that the syntrophic Geobacter aggregates are significantly different from their planktonic cells, indicating a distinct chemical composition (i.e., amino acids, fatty acids, and lipids) and structure formed on their surface. Among these characteristic components, amino acid fragments dominated in the variance, suggesting the importance of proteins in the coculture. Additionally, the quorum sensing signal molecule N-butyryl-L-homoserine lactone was observed in cocultured Geobacter aggregates, implying its role in syntrophic growth and aggregate formation. Furthermore, the electron acceptor organism G. sulfurreducens was shown to be the dominant species in syntrophic communities that drove the syntrophic growth. These results demonstrate that unique chemical compositions distinguish syntrophic Geobacter aggregates from planktonic cells and suggest that ToF-SIMS may be a promising tool to understand the syntrophic mechanism and investigate interspecies electron transfer pathways in complex biofilms. (C) 2017 American Vacuum Society.
研究领域[WOS]: Biophysics ; Materials Science
项目资助者: Pacific Northwest National Laboratory (PNNL) ; Earth and Biological Sciences Directorate (EBSD) mission seed Laboratory Directed Research and Development (LDRD) ; Yantai Institute of Coastal Zone Research Chinese Academy of Sciences ; National Natural Science Foundation of China(41371257, 41573071) ; Shandong Natural Science Fund for Distinguished Young Scholars(JQ201608) ; Young Taishan Scholars Program of Shandong Province(tsqn20161054) ; Office of Biological and Environmental Research(49694) ; PNNL
关键词[WOS]: ION MASS-SPECTROMETRY ; INTERSPECIES ELECTRON-TRANSFER ; ADSORBED PROTEIN FILMS ; C-TYPE CYTOCHROME ; PRINCIPAL COMPONENT ANALYSIS ; POLAR AMINO-ACIDS ; PSEUDOMONAS-AERUGINOSA ; MULTIVARIATE-ANALYSIS ; BIOFILM FORMATION ; OUTER-SURFACE
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000407944600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/23414
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China
2.Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Richland, WA 99354 USA
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
4.Pacific Northwest Natl Lab, Environm & Mol Sci Lab, Richland, WA 99352 USA
5.Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Washington, DC 99352 USA
6.Pacific Northwest Natl Lab, Energy & Environm Directorate, Richland, WA 99352 USA

Recommended Citation:
Wei, Wenchao,Zhang, Yanyan,Komorek, Rachel,et al. Characterization of syntrophic Geobacter communities using ToF-SIMS[J]. BIOINTERPHASES,2017,12(5).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wei, Wenchao]'s Articles
[Zhang, Yanyan]'s Articles
[Komorek, Rachel]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wei, Wenchao]‘s Articles
[Zhang, Yanyan]‘s Articles
[Komorek, Rachel]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院烟台海岸带研究所 - Feedback
Powered by CSpace