Characterization of syntrophic Geobacter communities using ToF-SIMS
Wei, Wenchao1,2,3; Zhang, Yanyan4; Komorek, Rachel5; Plymale, Andrew6; Yu, Rujia5; Wang, Bingchen1; Zhu, Zihua4; Liu, Fanghua1; Yu, Xiao-Ying2; Liu, Fanghua(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China); Yu, Xiao-Ying(Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Richland, WA 99354 USA)
2017-12-01
发表期刊BIOINTERPHASES
ISSN1934-8630
卷号12期号:5
产权排序第1完成单位
摘要The aggregation of syntrophic Geobacter metallireducens and Geobacter sulfurreducens is beneficial for enhancing direct interspecies electron transfer (DIET). Although DIET was suspected to occur on the microbial community surface, the surface chemical speciation of such cocultured communities remains unclear. In order to better understand surface interactions related to DIET, the authors characterized a series of samples associated with syntrophic G. metallireducens and G. sulfurreducens using surface sensitive time-of-flight secondary ion mass spectrometry (ToF-SIMS). Principal component analysis was used in spectral analysis. Our results show that the syntrophic Geobacter aggregates are significantly different from their planktonic cells, indicating a distinct chemical composition (i.e., amino acids, fatty acids, and lipids) and structure formed on their surface. Among these characteristic components, amino acid fragments dominated in the variance, suggesting the importance of proteins in the coculture. Additionally, the quorum sensing signal molecule N-butyryl-L-homoserine lactone was observed in cocultured Geobacter aggregates, implying its role in syntrophic growth and aggregate formation. Furthermore, the electron acceptor organism G. sulfurreducens was shown to be the dominant species in syntrophic communities that drove the syntrophic growth. These results demonstrate that unique chemical compositions distinguish syntrophic Geobacter aggregates from planktonic cells and suggest that ToF-SIMS may be a promising tool to understand the syntrophic mechanism and investigate interspecies electron transfer pathways in complex biofilms. (C) 2017 American Vacuum Society.
DOI10.1116/1.4986832
项目资助者Pacific Northwest National Laboratory (PNNL) ; Earth and Biological Sciences Directorate (EBSD) mission seed Laboratory Directed Research and Development (LDRD) ; Yantai Institute of Coastal Zone Research Chinese Academy of Sciences ; National Natural Science Foundation of China(41371257, 41573071) ; Shandong Natural Science Fund for Distinguished Young Scholars(JQ201608) ; Young Taishan Scholars Program of Shandong Province(tsqn20161054) ; Office of Biological and Environmental Research(49694) ; PNNL
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收录类别SCI
关键词[WOS]ION MASS-SPECTROMETRY ; INTERSPECIES ELECTRON-TRANSFER ; ADSORBED PROTEIN FILMS ; C-TYPE CYTOCHROME ; PRINCIPAL COMPONENT ANALYSIS ; POLAR AMINO-ACIDS ; PSEUDOMONAS-AERUGINOSA ; MULTIVARIATE-ANALYSIS ; BIOFILM FORMATION ; OUTER-SURFACE
文章类型Article
语种英语
WOS研究方向Biophysics ; Materials Science
WOS记录号WOS:000407944600001
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/23414
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Liu, Fanghua; Yu, Xiao-Ying; Liu, Fanghua(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China); Yu, Xiao-Ying(Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Richland, WA 99354 USA)
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China
2.Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Richland, WA 99354 USA
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
4.Pacific Northwest Natl Lab, Environm & Mol Sci Lab, Richland, WA 99352 USA
5.Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Washington, DC 99352 USA
6.Pacific Northwest Natl Lab, Energy & Environm Directorate, Richland, WA 99352 USA
第一作者单位中国科学院烟台海岸带研究所
通讯作者单位中国科学院烟台海岸带研究所
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Wei, Wenchao,Zhang, Yanyan,Komorek, Rachel,et al. Characterization of syntrophic Geobacter communities using ToF-SIMS[J]. BIOINTERPHASES,2017,12(5).
APA Wei, Wenchao.,Zhang, Yanyan.,Komorek, Rachel.,Plymale, Andrew.,Yu, Rujia.,...&Yu, Xiao-Ying.(2017).Characterization of syntrophic Geobacter communities using ToF-SIMS.BIOINTERPHASES,12(5).
MLA Wei, Wenchao,et al."Characterization of syntrophic Geobacter communities using ToF-SIMS".BIOINTERPHASES 12.5(2017).
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