Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing
Sun, Lina1; Sun, Jingchun1; Li, Xiaoni1,2; Zhang, Libin1; Yang, Hongsheng2; Wang, Qing3
2017-06-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN1744-117X
卷号22页码:1-9
摘要The sea cucumber, as a member of the Echinodermata, has the capacity to restore damaged organs and body parts, which has always been a key scientific issue. MicroRNAs (miRNAs), a class of short noncoding RNAs, play important roles in regulating gene expression. In the present study, we applied high-throughput sequencing to investigate alterations of miRNA expression in regenerative intestine compared to normal intestine. A total of 73 differentially expressed miRNAs were obtained, including 59 up-regulated miRNAs and 14 down-regulated miRNAs. Among these molecules, Aja-miR-1715-5p, Aja-miR-153, Aja-miR-252a, Aja-miR-153-5p, Aja-miR-252b, Aja-miR-2001, Aja-miR-64d-3p, and Aja-miR-252-5p were differentially expressed over 10-fold at 3 days post-evisceration (dpe). Notably, real-time PCR revealed that Aja-miR-1715-5p was up-regulated 1390 fold at 3 dpe. Moreover, putative target gene co-expression analyses, gene ontology, and pathway analyses suggest that these miRNAs play important roles in specific cellular events (cell proliferation, migration, and apoptosis), metabolic regulation, and energy redistribution. These results will provide a basis for future studies of miRNA regulation in sea cucumber regeneration. (C) 2017 Elsevier Inc. All rights reserved.
关键词Sea Cucumber Micrornas Mirna Expression Profiles Intestine Regeneration
DOI10.1016/j.cbd.2017.01.001
项目资助者National Natural Science Foundation of China(41406168) ; Science and Technology Innovation Plan of Aoshan(2015ASKJ02-05) ; Chinese Academy of Sciences(XDA11020703) ; Key Laboratory of Marine Ecology and the Environmental Science, Institute of Oceanology, Chinese Academy of Sciences(KLMEES201301)
收录类别SCI
关键词[WOS]HOLOTHURIA-GLABERRIMA ; CELL-PROLIFERATION ; DOWN-REGULATION ; VISCERAL REGENERATION ; EXPRESSION ; GENE ; CANCER ; DROSOPHILA ; MIR-133 ; DEDIFFERENTIATION
文章类型Article
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号WOS:000402220500001
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22511
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remedia, Yantai, Peoples R China
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Sun, Lina,Sun, Jingchun,Li, Xiaoni,et al. Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2017,22:1-9.
APA Sun, Lina,Sun, Jingchun,Li, Xiaoni,Zhang, Libin,Yang, Hongsheng,&Wang, Qing.(2017).Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,22,1-9.
MLA Sun, Lina,et al."Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 22(2017):1-9.
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