Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing
Sun, Lina1; Sun, Jingchun1; Li, Xiaoni1,2; Zhang, Libin1; Yang, Hongsheng2; Wang, Qing3
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN1744-117X
2017-06-01
卷号22页码:1-9
关键词Sea Cucumber Micrornas Mirna Expression Profiles Intestine Regeneration
研究领域Biochemistry & Molecular Biology ; Genetics & Heredity
DOI10.1016/j.cbd.2017.01.001
产权排序[Sun, Lina; Sun, Jingchun; Li, Xiaoni; Zhang, Libin] Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China; [Li, Xiaoni; Yang, Hongsheng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Wang, Qing] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remedia, Yantai, Peoples R China
作者部门海岸带生物资源高效利用研究与发展中心
英文摘要The sea cucumber, as a member of the Echinodermata, has the capacity to restore damaged organs and body parts, which has always been a key scientific issue. MicroRNAs (miRNAs), a class of short noncoding RNAs, play important roles in regulating gene expression. In the present study, we applied high-throughput sequencing to investigate alterations of miRNA expression in regenerative intestine compared to normal intestine. A total of 73 differentially expressed miRNAs were obtained, including 59 up-regulated miRNAs and 14 down-regulated miRNAs. Among these molecules, Aja-miR-1715-5p, Aja-miR-153, Aja-miR-252a, Aja-miR-153-5p, Aja-miR-252b, Aja-miR-2001, Aja-miR-64d-3p, and Aja-miR-252-5p were differentially expressed over 10-fold at 3 days post-evisceration (dpe). Notably, real-time PCR revealed that Aja-miR-1715-5p was up-regulated 1390 fold at 3 dpe. Moreover, putative target gene co-expression analyses, gene ontology, and pathway analyses suggest that these miRNAs play important roles in specific cellular events (cell proliferation, migration, and apoptosis), metabolic regulation, and energy redistribution. These results will provide a basis for future studies of miRNA regulation in sea cucumber regeneration. (C) 2017 Elsevier Inc. All rights reserved.
文章类型Article
资助机构National Natural Science Foundation of China(41406168) ; Science and Technology Innovation Plan of Aoshan(2015ASKJ02-05) ; Chinese Academy of Sciences(XDA11020703) ; Key Laboratory of Marine Ecology and the Environmental Science, Institute of Oceanology, Chinese Academy of Sciences(KLMEES201301)
收录类别SCI
语种英语
关键词[WOS]HOLOTHURIA-GLABERRIMA ; CELL-PROLIFERATION ; DOWN-REGULATION ; VISCERAL REGENERATION ; EXPRESSION ; GENE ; CANCER ; DROSOPHILA ; MIR-133 ; DEDIFFERENTIATION
研究领域[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号WOS:000402220500001
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22511
专题中国科学院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remedia, Yantai, Peoples R China
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Sun, Lina,Sun, Jingchun,Li, Xiaoni,et al. Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2017,22:1-9.
APA Sun, Lina,Sun, Jingchun,Li, Xiaoni,Zhang, Libin,Yang, Hongsheng,&Wang, Qing.(2017).Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,22,1-9.
MLA Sun, Lina,et al."Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 22(2017):1-9.
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