The present invention discloses a petroleum degrading strain CN-3, which is preserved in China Center For Type Culture Collection, and the accession number is CCTCC M 2015537. A screening medium and a degradation medium used in experiments both use petroleum as a sole carbon source and sole energy, and the screened-out CN-3 strain is highly efficient in petroleum degradation. The petroleum degrading strain CN-3 has good petroleum degradation effect in the neutral to slightly alkaline environment, can withstand high salinity environment, and is especially suitable for the marine environment, so that the range of application of the petroleum degrading strain CN-3 in marine oil pollution treatment is expanded, and the petroleum degrading strain CN-3 has more application prospects.
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