The invention provides a petroleum hydrocarbon degrading strain, namely, Acinetobacter venetianus LCL-1. According to the petroleum hydrocarbon degrading strain, the ocean bottom mud polluted by petroleum is screened to obtain ocean indigenous microbes which can adapt to the complex ocean environment and efficiently degrade petroleum hydrocarbon, the commercial value is good, and the application is wide. In addition, the invention further provides a preparation method of polyurethane foam of petroleum hydrocarbon degrading bacteria; the preparation method comprises the following steps : dispersing polyurethane foam in deionized water, adjusting the pH, adding with diethylenetriamine for water bath reaction, carrying out water washing, carrying out vacuum drying, thus obtaining amine modified polyurethane foam, dispersing the amine modified polyurethane foam in an LB culture medium, inoculating the strain into the LB culture medium, carrying out cross-linking reaction, thus obtaining the polyurethane foam of the petroleum hydrocarbon degrading bacteria; the amine modified foam has cross-linking reaction with extracelluar secreta on the surfaces of the microbes, so that immobilization and apposition growth of the microbes are facilitated, the immobilized microbes can be protected from being washed away by seawater, so that the recycling is facilitated.
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