Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2 | |
Hu, Xiaoke1; Wang, Caixia1,2; Wang, Peng3 | |
发表期刊 | FRONTIERS IN MICROBIOLOGY |
ISSN | 1664-302X |
2015-09-28 | |
卷号 | 6 |
关键词 | Microbial Biosurfactant Optimal Production Surface Tension Vibrio Alginolyticus Glycoproteins |
DOI | 10.3389/fmicb.2015.00976 |
英文摘要 | A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28 degrees C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation. |
文章类型 | Article |
资助机构 | project of on-site sediment microbial remediation of public area of central Bohai Sea, North China Sea Branch of State Oceanic Administration(QDZC20150420-002) ; National Natural Science Foundation of China(41376138) ; Hundred Talents Program of Chinese Academy of Sciences |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | ANALYTICAL HIERARCHY PROCESS ; CRUDE-OIL ; BIOEMULSIFIER ; HYDROCARBON ; BACTERIA ; MICROORGANISMS ; FERMENTATION ; SURFACTANTS ; CULTURE ; FIBERS |
研究领域[WOS] | Microbiology |
WOS记录号 | WOS:000378319700001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/17091 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Chunhui Rd, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Qingdao, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, Xiaoke,Wang, Caixia,Wang, Peng. Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2[J]. FRONTIERS IN MICROBIOLOGY,2015,6. |
APA | Hu, Xiaoke,Wang, Caixia,&Wang, Peng.(2015).Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2.FRONTIERS IN MICROBIOLOGY,6. |
MLA | Hu, Xiaoke,et al."Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2".FRONTIERS IN MICROBIOLOGY 6(2015). |
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