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藻类特有的捕光色素蛋白——藻红蛋白的结构、功能及应用 期刊论文
科学通报, 2020, 卷号: 65, 期号: 07, 页码: 565-576
作者:  臧帆;  秦松;  马丞博;  李文军;  林剑
Adobe PDF(1397Kb)  |  收藏  |  浏览/下载：749/241  |  提交时间：2020/07/08
藻红蛋白  结构功能  制备工艺  光学活性  生物活性  
藻类特有的捕光色素蛋白藻红蛋白的结构、功能及应用 期刊论文
科学通报, 2020, 卷号: 65, 期号: 7, 页码: 565-576
作者:  臧帆;  秦松;  马丞博;  李文军;  林剑
Adobe PDF(1396Kb)  |  收藏  |  浏览/下载：198/51  |  提交时间：2021/12/01
藻红蛋白  结构功能  制备工艺  光学活性  生物活性  
藻胆蛋白生物合成研究进展 期刊论文
科学通报, 2019, 卷号: 64, 期号: 01, 页码: 49-59
作者:  马丞博;  秦松;  李文军;  葛保胜
Adobe PDF(12113Kb)  |  收藏  |  浏览/下载：466/76  |  提交时间：2020/07/08
藻胆蛋白  生物合成  体外重组  抗氧化  荧光  
食用蔬菜能吸收和积累微塑料 期刊论文
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:  李连祯;  周倩;  尹娜;  涂晨;  骆永明
收藏  |  浏览/下载：520/0  |  提交时间：2020/06/17
微塑料  生菜  聚苯乙烯微球  吸收  积累  健康风险  microplastics  lettuce  polystyrene microbeads  uptake  accumulation  human health risk  Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.  
分子印迹材料的先进制备技术与策略 期刊论文
科学通报, 2019, 卷号: 64, 期号: 13, 页码: 1352-1367
作者:  王晓艳;  李金花;  陈令新
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分子印迹聚合物  印迹技术  印迹策略  样品前处理  传感  刺激响应印迹  Molecularly Imprinted Polymers  Imprinting Technology  Imprinting Strategy  Sample Pretreatment  Sensor  Stimuliresponsive Imprinting  
钝顶螺旋藻C-藻蓝蛋白的能量传递动力学 期刊论文
科学通报, 2018, 卷号: 63, 期号: 07, 页码: 641-648
作者:  王肖肖;  李文军;  谢明远;  赵福利;  杨革;  刘琪;  秦松
Adobe PDF(1583Kb)  |  收藏  |  浏览/下载：332/72  |  提交时间：2020/07/08
C-藻蓝蛋白六聚体  时间分辨光谱  能量传递  
重组别藻蓝蛋白三聚体结构与功能 期刊论文
科学通报, 2017, 卷号: 62, 期号: 16, 页码: 1699-1713
作者:  李文军;  蒲洋;  牛壮;  薛春岭;  秦松
收藏  |  浏览/下载：196/0  |  提交时间：2020/07/07
recombinant allophycocyanin trimer  exciton-coupled pairs  absorption spectrum  fluorescence spectrum  circular dichroism spectra  conformational stability  重组别藻蓝蛋白三聚体  激子耦合对  吸收光谱  荧光光谱  圆二色谱  构象稳定性  
化学品甲状腺干扰效应的计算毒理学研究进展 期刊论文
科学通报, 2015, 卷号: 60, 期号: 19, 页码: 1761-1771
作者:  杨先海;  陈景文;  李斐
浏览  |  Adobe PDF(1970Kb)  |  收藏  |  浏览/下载：811/343  |  提交时间：2016/12/15
甲状腺干扰效应  甲状腺干扰物  计算毒理学  分子模拟  定量构效关系  
典型持久性有机污染物与抑癌基因相互作用的分子模拟与验证 期刊论文
科学通报, 2015, 卷号: 60, 期号: 19, 页码: 1804-1809
作者:  吴惠丰;  曹璐璐;  李斐;  李雪花;  赵建民
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持久性有机污染物  P53  2  分子模拟  2'  光谱法  4  4'-四溴联苯醚(Bde-47)