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题名:
Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene
作者: Shu, Kun-xian3,5; Li, Yan-zhe3; Shao, HongBo(邵宏波)1,2,4,6; Zhang, Ming-hua5; Wu, Li-xiang5
刊名: AFRICAN JOURNAL OF BIOTECHNOLOGY
出版日期: 2010-08-16
卷号: 9, 期号:33, 页码:5262-5268
关键词: p53 Gene ; p53 downstream gene ; cDNA library ; normalization
英文摘要: The tumor-suppressor gene p53 and its downstream genes consist of a complicated gene network, and the challenge to understand the network is to identify p53 downstream genes. In order to isolate and identify new p53 regulated genes, we constructed and characterized a normalized cDNA library from human brain glioma cell line U251 while exogenous p53 gene is overexpressed. The constructed cDNA library contained 1.3x10(6) directional recombinants, and its insert size ranged from 0.5 to 2.0 kb. Screening the cDNA library, we obtained two novel p53 downstream genes, PAP1 and PAP2. Polymerase chain reaction (PCR) analyses of the library for specific genes revealed the presence of cDNAs for p53 downstream genes such as p21, gadd45, and PCNA. These results demonstrate the sequence complexity and relatively low redundancy of our cDNA library. It is a valuable and unique resource for studying p53 gene expression, regulatory mechanisms and screening p53 downstream genes.
研究领域[WOS]: Biotechnology & Applied Microbiology
关键词[WOS]: SIZE FRACTIONATION ; PCR AMPLIFICATION ; DOWNSTREAM GENES ; HUMAN GENOME ; RNA ; EXPRESSION ; NETWORK ; CLONES
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000281756000003
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内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/9234
Appears in Collections:滨海湿地实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
2.Minist Water Resources, Yangling 712100, Peoples R China
3.Chongqing Univ Posts & Telecommun, Coll Bioinformat, Chongqing 400065, Peoples R China
4.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China
5.Cent S Univ, Xiangya Sch Med, Changsha 410008, Hunan, Peoples R China
6.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China

Recommended Citation:
Shu, Kun-xian,Li, Yan-zhe,Shao, Hong-bo,et al. Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(33):5262-5268.
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