Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene
Shu, Kun-xian3,5; Li, Yan-zhe3; Shao, Hong-bo1,2,4,6; Zhang, Ming-hua5; Wu, Li-xiang5
2010-08-16
Source PublicationAFRICAN JOURNAL OF BIOTECHNOLOGY
Volume9Issue:33Pages:5262-5268
AbstractThe tumor-suppressor gene p53 and its downstream genes consist of a complicated gene network, and the challenge to understand the network is to identify p53 downstream genes. In order to isolate and identify new p53 regulated genes, we constructed and characterized a normalized cDNA library from human brain glioma cell line U251 while exogenous p53 gene is overexpressed. The constructed cDNA library contained 1.3x10(6) directional recombinants, and its insert size ranged from 0.5 to 2.0 kb. Screening the cDNA library, we obtained two novel p53 downstream genes, PAP1 and PAP2. Polymerase chain reaction (PCR) analyses of the library for specific genes revealed the presence of cDNAs for p53 downstream genes such as p21, gadd45, and PCNA. These results demonstrate the sequence complexity and relatively low redundancy of our cDNA library. It is a valuable and unique resource for studying p53 gene expression, regulatory mechanisms and screening p53 downstream genes.
KeywordP53 Gene P53 Downstream Gene Cdna Library Normalization
Indexed BySCI
WOS KeywordSIZE FRACTIONATION ; PCR AMPLIFICATION ; DOWNSTREAM GENES ; HUMAN GENOME ; RNA ; EXPRESSION ; NETWORK ; CLONES
SubtypeArticle
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS IDWOS:000281756000003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/9234
Collection中科院海岸带环境过程与生态修复重点实验室_滨海湿地实验室
Affiliation1.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
2.Minist Water Resources, Yangling 712100, Peoples R China
3.Chongqing Univ Posts & Telecommun, Coll Bioinformat, Chongqing 400065, Peoples R China
4.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China
5.Cent S Univ, Xiangya Sch Med, Changsha 410008, Hunan, Peoples R China
6.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China
Recommended Citation
GB/T 7714
Shu, Kun-xian,Li, Yan-zhe,Shao, Hong-bo,et al. Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(33):5262-5268.
APA Shu, Kun-xian,Li, Yan-zhe,Shao, Hong-bo,Zhang, Ming-hua,&Wu, Li-xiang.(2010).Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(33),5262-5268.
MLA Shu, Kun-xian,et al."Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.33(2010):5262-5268.
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