Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene
Shu, Kun-xian3,5; Li, Yan-zhe3; Shao, Hong-bo1,2,4,6; Zhang, Ming-hua5; Wu, Li-xiang5
2010-08-16
发表期刊AFRICAN JOURNAL OF BIOTECHNOLOGY
卷号9期号:33页码:5262-5268
摘要The tumor-suppressor gene p53 and its downstream genes consist of a complicated gene network, and the challenge to understand the network is to identify p53 downstream genes. In order to isolate and identify new p53 regulated genes, we constructed and characterized a normalized cDNA library from human brain glioma cell line U251 while exogenous p53 gene is overexpressed. The constructed cDNA library contained 1.3x10(6) directional recombinants, and its insert size ranged from 0.5 to 2.0 kb. Screening the cDNA library, we obtained two novel p53 downstream genes, PAP1 and PAP2. Polymerase chain reaction (PCR) analyses of the library for specific genes revealed the presence of cDNAs for p53 downstream genes such as p21, gadd45, and PCNA. These results demonstrate the sequence complexity and relatively low redundancy of our cDNA library. It is a valuable and unique resource for studying p53 gene expression, regulatory mechanisms and screening p53 downstream genes.
关键词P53 Gene P53 Downstream Gene Cdna Library Normalization
收录类别SCI
关键词[WOS]SIZE FRACTIONATION ; PCR AMPLIFICATION ; DOWNSTREAM GENES ; HUMAN GENOME ; RNA ; EXPRESSION ; NETWORK ; CLONES
文章类型Article
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS记录号WOS:000281756000003
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/9234
专题中科院海岸带环境过程与生态修复重点实验室_滨海湿地实验室
作者单位1.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
2.Minist Water Resources, Yangling 712100, Peoples R China
3.Chongqing Univ Posts & Telecommun, Coll Bioinformat, Chongqing 400065, Peoples R China
4.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China
5.Cent S Univ, Xiangya Sch Med, Changsha 410008, Hunan, Peoples R China
6.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China
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Shu, Kun-xian,Li, Yan-zhe,Shao, Hong-bo,et al. Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(33):5262-5268.
APA Shu, Kun-xian,Li, Yan-zhe,Shao, Hong-bo,Zhang, Ming-hua,&Wu, Li-xiang.(2010).Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(33),5262-5268.
MLA Shu, Kun-xian,et al."Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.33(2010):5262-5268.
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