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Alternative TitleEffects of Salt Stress on Photosynthetic Characters in Honeysuckle with Different Ploidies
颜坤1; 赵世杰; 徐化凌; 吴从稳; 陈小兵1
Source Publication中国农业科学
Keyword金银花 光系统 光合电子传递 气孔限制 羧化效率
DOI10.3864 / j.issn.0578-1752.2015.16.017

【目的】研究四倍体和二倍体金银花叶片光合作用对盐胁迫的响应,特别是盐胁迫对PSⅠ和PSⅡ性能以及协调性的影响,比较盐胁迫下叶片光合特性的差异,分析盐胁迫对叶片Na+、Cl-和丙二醛含量以及叶片生物量的影响,揭示不同倍性金银花耐盐胁迫的能力,为盐碱地栽培品种的选择提供参考。【方法】选用四倍体和二倍体金银花为试验材料,采用叶绿素荧光快速诱导动力学曲线和820 nm光反射曲线同步测定技术,结合气体交换参数,研究中度(150 mmol·L-1 Na Cl)和重度(300 mmol·L-1 Na Cl)盐胁迫对四倍体和二倍体金银花叶片光合作用和光合机构的影响。金银花植株定植于装有石英砂的塑料盆中,Hoa...;【Objective】In order to provide a reference for cultivar selection of saline land, leaf photosynthetic responses to salt stress in diploid and tetraploid honeysuckle, especially the effects of salt stress on PS and PS performance and coordination wer Ⅰ Ⅱ e studied, the difference in leaf photosynthetic characters under salt stress was compared and the effects of salt stress on leaf Na+ , Cland malondialdehyde contents and the leaf biomass were analyzed so that revealed salt tolerance of the honeysuckle with different ploidies.【Method】Tetraploid and diploid honeysuckle plants were used as experimental materials to study the effects of moderate (150 mmol·L-1 NaCl) and severe (300 mmol·L-1 NaCl) salt stress on photosynthesis and photosynthetic apparatus by simultaneously analyzing chlorophyll a fluorescence transient and light reflection at 820 nm as well as gas exchange parameters. Honeysuckle plants were planted in plastic pots filled with quartz sand and cultured with Hoagland nutrient solution. NaCl was added to nutrient solution incrementally by 50 mmol·L-1 step per day to provide final concentrations of 150 and 300 mmol·L-1 for salt treatment, and the treatment lasted for 15 days. The control plants were cultured with nutrient solution without adding NaCl. During salt treatment, expanded leaves from the middle of a shoot were sampled for measuring parameters.【Result】Under moderate salt stress, leaf photosynthetic rate, stomatal conductance and intercellular CO2 concentration were significantly decreased in diploid and tetraploid honeysuckle, and the decrease was lower in the tetraploid, indicating less stomatal inhibition on photosynthesis. Under severe salt stress, leaf photosynthetic rate was also significantly decreased in diploid and tetraploid honeysuckle, and the tetraploid could maintain higher photosynthetic activity than the diploid due to the lower decrease in leaf photosynthetic rate. After 7 days of severe salt stress, leaf carboxylation rate and Rubisco activation state were significantly decreased in diploid honeysuckle. PS photoinhibition was induced, resulting in the decline of quantum Ⅱ yield of electron transport to PS . Therefore, PS reduction Ⅰ Ⅰ was inhibited, and the increased PSⅠoxidation should be observed in the 820 nm reflection transient. On the contrary, PS oxidation was significantly decreased. Unde Ⅰ Ⅰ r severe salt stress, PS photoinhibition also occurred in the leaves of diploid honeysuckle, which could not effectively drive electrons to its acceptor side and then inhibited PS oxidation. The sign Ⅰ ificant decrease of PS oxidation resulted from the greater photoinhibition in PS than PS , and the coordination between PS and Ⅰ ⅠⅡ Ⅱ PS was destroyed. After 15 days of severe Ⅰ salt stress, leaf carboxylation rate and activation state of Rubisco enzyme were significantly decreased in tetraploid honeysuckle, and the decrease was lower than that in diploid honeysuckle. The capacity of PS and PS was not significantly affected, and Ⅱ Ⅰ their coordination was maintained in tetraploid honeysuckle under severe salt stress. Thus, tetraploid honeysuckle also had higher leaf photosynthetic activity than the diploid upon severe salt stress. After salt stress for 15 days, leaf Na+ , Cl- and malondialdehyde contents were significantly increased in diploid honeysuckle, whereas leaf dry weight per plant was significantly reduced, and the change amplitude was greater than that in tetraploid honeysuckle, indicating lower leaf ionic toxicity in the tetraploid, which might be responsible for maintaining higher photosynthetic capacity under salt stress.【Conclusion】 Under salt stress, tetraploid honeysuckle possesses higher leaf photosynthetic activity in terms of greater leaf stomatal conductance, higher Rubisco activation state and normal PSⅡ and PS coordination, and then c Ⅰ ould accumulate greater biomass. Therefore, tetraploid honeysuckle has stronger salt tolerance and is more suitable for cultivation in saline land.

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颜坤,赵世杰,徐化凌,等. 盐胁迫对不同倍性金银花光合特性的影响[J]. 中国农业科学,2015,48(16):3275-3286.
APA 颜坤,赵世杰,徐化凌,吴从稳,&陈小兵.(2015).盐胁迫对不同倍性金银花光合特性的影响.中国农业科学,48(16),3275-3286.
MLA 颜坤,et al."盐胁迫对不同倍性金银花光合特性的影响".中国农业科学 48.16(2015):3275-3286.
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