| Use of bacteria-immobilized cotton fibers to absorb and degrade crude oil | |
Lin, Meng1; Liu, Yuhua1,2; Chen, Weiwei1,2; Wang, Hui1 ; Hu, Xiaoke1; Hu, XK (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China. xkhu@yic.ac.cn
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| 发表期刊 | INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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| ISSN | 0964-8305 |
| 2014-03-01 | |
| 卷号 | 88页码:8-12 |
| 关键词 | Biodegradation Immobilization Crude Oil Cotton Fibers Saturated Hydrocarbons |
| DOI | 10.1016/j.ibiod.2013.11.015 |
| 产权排序 | [Lin, Meng; Liu, Yuhua; Chen, Weiwei; Wang, Hui; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Liu, Yuhua; Chen, Weiwei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Using enrichment culture technique, two isolates that brought a significant degradation and dispersion of crude oil were obtained from contaminated sediments of the Bohai Bay, China. 16S rRNA gene sequencing and phylogenetic analysis indicated that the two bacterial strains affiliated with the genera Vibrio and Acinetobacter. Subsequently, the bacterial cells were immobilized on the surface of cotton fibers. Cotton fibers were used as crude oil sorbent as well as a biocarrier for bacteria immobilization. Among the two isolates, the marine bacteria Acinetobacter sp. HC8-3S showed a strong binding to the cotton fibers, possibly enhanced through extracellular dispersant excreted by Acinetobacter sp. HC8-3S. Both planktonic and immobilized bacteria showed relatively high biodegradation (>60%) of saturated hydrocarbons fraction of crude oil, in the pH range of 5.6-8.6. The degradation activities of planktonic and immobilized bacteria were not affected significantly when the NaCl concentration reached 70 g/L. The immobilized bacterial cells exhibited an enhanced biodegradation of crude oil. The efficiency of saturated hydrocarbons degradation by the immobilized bacterial cells increased about 30% compared to the planktonic bacterial cells. (C) 2013 Elsevier Ltd. All rights reserved.; Using enrichment culture technique, two isolates that brought a significant degradation and dispersion of crude oil were obtained from contaminated sediments of the Bohai Bay, China. 16S rRNA gene sequencing and phylogenetic analysis indicated that the two bacterial strains affiliated with the genera Vibrio and Acinetobacter. Subsequently, the bacterial cells were immobilized on the surface of cotton fibers. Cotton fibers were used as crude oil sorbent as well as a biocarrier for bacteria immobilization. Among the two isolates, the marine bacteria Acinetobacter sp. HC8-3S showed a strong binding to the cotton fibers, possibly enhanced through extracellular dispersant excreted by Acinetobacter sp. HC8-3S. Both planktonic and immobilized bacteria showed relatively high biodegradation (>60%) of saturated hydrocarbons fraction of crude oil, in the pH range of 5.6-8.6. The degradation activities of planktonic and immobilized bacteria were not affected significantly when the NaCl concentration reached 70 g/L. The immobilized bacterial cells exhibited an enhanced biodegradation of crude oil. The efficiency of saturated hydrocarbons degradation by the immobilized bacterial cells increased about 30% compared to the planktonic bacterial cells. (C) 2013 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | HYDROCARBON DEGRADATION ; BIODEGRADATION ; BIOREMEDIATION ; CHITOSAN ; MICROORGANISMS ; ADSORPTION ; SURFACE ; CELLS ; WATER |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| WOS记录号 | WOS:000331922000002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8558 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Hu, XK (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China. xkhu@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Meng,Liu, Yuhua,Chen, Weiwei,et al. Use of bacteria-immobilized cotton fibers to absorb and degrade crude oil[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2014,88:8-12. |
| APA | Lin, Meng,Liu, Yuhua,Chen, Weiwei,Wang, Hui,Hu, Xiaoke,&Hu, XK .(2014).Use of bacteria-immobilized cotton fibers to absorb and degrade crude oil.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,88,8-12. |
| MLA | Lin, Meng,et al."Use of bacteria-immobilized cotton fibers to absorb and degrade crude oil".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 88(2014):8-12. |
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