A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo

YIC-IR > 中国科学院海岸带环境过程与生态修复重点实验室 > 海岸带环境工程技术研究与发展中心

	A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo
	Hu, Yunxia; Haynes, Matthew T.; Wang, Yuhua; Liu, Feng; Huang, Leaf; Hu, YX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chun Hui Rd, Yantai 264003, Shandong, Peoples R China. yunxiahu@yic.ac.cn; leafh@unc.edu
发表期刊	ACS NANO
ISSN	1936-0851
	2013-06
卷号	7 期号:6 页码:5376-5384
关键词	Nanoparticle Liposome Liver Hepatocyte Dna Delivery Arginine-rich Peptide
产权排序	[Hu, Yunxia; Haynes, Matthew T.; Wang, Yuhua; Liu, Feng; Huang, Leaf] Univ N Carolina, Eshelman Sch Pharm, Div Mol Pharmaceut, Ctr Nanotechnol Drug Delivery, Chapel Hill, NC 27599 USA
英文摘要	Multifunctional membrane-core nanoparticles, composed of calcium phosphate cores, arginine-rich peptides, cationic and PEGylated lipid membranes, and galactose targeting ligands, have been developed as synthetic vectors for efficient nuclear delivery of plasmid DNA and subsequent gene expression in hepatocytes in vivo. Targeted particles exhibited rapid and extensive hepatic accumulation and were predominantly internalized by hepatocytes, while the Inclusion of such peptides in Le was sufficient to elicit high degrees of nuclear translocation of plasmid DNA. Monocyclic CR8C significantly enhanced In vivo gene expression over 10-fold more than linear CR8C, likely due to a release-favoring mechanism of the DNA/peptide complex. Though 100-fold lower in activity than that achieved via hydrodynamic injection, this formulation presents as a much less invasive alternative. To our knowledge, this is the most effective synthetic vector for liver gene transfer.
资助机构	NIH [CA129835, CA149363, CA125273, CA151652]
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/7058
专题	中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Hu, YX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chun Hui Rd, Yantai 264003, Shandong, Peoples R China. yunxiahu@yic.ac.cn; leafh@unc.edu
推荐引用方式 GB/T 7714	Hu, Yunxia,Haynes, Matthew T.,Wang, Yuhua,et al. A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo[J]. ACS NANO,2013,7(6):5376-5384.
APA	Hu, Yunxia.,Haynes, Matthew T..,Wang, Yuhua.,Liu, Feng.,Huang, Leaf.,...&leafh@unc.edu.(2013).A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo.ACS NANO,7(6),5376-5384.
MLA	Hu, Yunxia,et al."A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo".ACS NANO 7.6(2013):5376-5384.

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