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A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo
Hu, Yunxia; Haynes, Matthew T.; Wang, Yuhua; Liu, Feng; Huang, Leaf; Hu, YX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chun Hui Rd, Yantai 264003, Shandong, Peoples R China. yunxiahu@yic.ac.cn; leafh@unc.edu
2013-06
发表期刊ACS NANO
ISSN1936-0851
卷号7期号:6页码:5376-5384
产权排序[Hu, Yunxia; Haynes, Matthew T.; Wang, Yuhua; Liu, Feng; Huang, Leaf] Univ N Carolina, Eshelman Sch Pharm, Div Mol Pharmaceut, Ctr Nanotechnol Drug Delivery, Chapel Hill, NC 27599 USA
摘要Multifunctional membrane-core nanoparticles, composed of calcium phosphate cores, arginine-rich peptides, cationic and PEGylated lipid membranes, and galactose targeting ligands, have been developed as synthetic vectors for efficient nuclear delivery of plasmid DNA and subsequent gene expression in hepatocytes in vivo. Targeted particles exhibited rapid and extensive hepatic accumulation and were predominantly internalized by hepatocytes, while the Inclusion of such peptides in Le was sufficient to elicit high degrees of nuclear translocation of plasmid DNA. Monocyclic CR8C significantly enhanced In vivo gene expression over 10-fold more than linear CR8C, likely due to a release-favoring mechanism of the DNA/peptide complex. Though 100-fold lower in activity than that achieved via hydrodynamic injection, this formulation presents as a much less invasive alternative. To our knowledge, this is the most effective synthetic vector for liver gene transfer.
关键词Nanoparticle Liposome Liver Hepatocyte Dna Delivery Arginine-rich Peptide
学科领域Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
项目资助者NIH [CA129835, CA149363, CA125273, CA151652]
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收录类别SCI
语种英语
资助项目海岸带环境工程技术研究中心
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7058
专题山东省海岸带环境工程技术研究中心
通讯作者Hu, YX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chun Hui Rd, Yantai 264003, Shandong, Peoples R China. yunxiahu@yic.ac.cn; leafh@unc.edu
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Hu, Yunxia,Haynes, Matthew T.,Wang, Yuhua,et al. A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo[J]. ACS NANO,2013,7(6):5376-5384.
APA Hu, Yunxia.,Haynes, Matthew T..,Wang, Yuhua.,Liu, Feng.,Huang, Leaf.,...&leafh@unc.edu.(2013).A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo.ACS NANO,7(6),5376-5384.
MLA Hu, Yunxia,et al."A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo".ACS NANO 7.6(2013):5376-5384.
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