A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane
Rotaru, Amelia-Elena; Shrestha, Pravin Malta; Liu, Fanghua; Shrestha, Minita; Shrestha, Devesh; Embree, Mallory; Zengler, Karsten; Wardman, Colin; Nevin, Kelly P.; Lovley, Derek R.; Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@microbio.umass.edu
2014-01
发表期刊ENERGY & ENVIRONMENTAL SCIENCE
ISSN1754-5692
卷号7期号:1页码:408-415
产权排序[Rotaru, Amelia-Elena; Shrestha, Pravin Malta; Liu, Fanghua; Shrestha, Minita; Shrestha, Devesh; Wardman, Colin; Nevin, Kelly P.; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA; [Liu, Fanghua] Yantai Inst Coastal Res, Yantai, Peoples R China; [Embree, Mallory; Zengler, Karsten] Univ Calif San Diego, La Jolla, CA 92093 USA
摘要Anaerobic conversion of organic wastes and biomass to methane is an important bioenergy strategy, which depends on poorly understood mechanisms of interspecies electron transfer to methanogenic microorganisms. Metatranscriptomic analysis of methanogenic aggregates from a brewery wastewater digester, coupled with fluorescence in situ hybridization with specific 16S rRNA probes, revealed that Methanosaeta species were the most abundant and metabolically active methanogens. Methanogens known to reduce carbon dioxide with H2 or formate as the electron donor were rare. Although Methanosaeta have previously been thought to be restricted to acetate as a substrate for methane production, Methanosaeta in the aggregates had a complete complement of genes for the enzymes necessary for the reduction of carbon to methane, and transcript abundance for these genes was high. Furthermore, Geobacter species, the most abundant bacteria in the aggregates, highly expressed genes for ethanol metabolism and for extracellular electron transfer via electrically conductive pili, suggesting that Geobacter and Methanosaeta species were exchanging electrons via direct interspecies electron transfer (DIET). This possibility was further investigated in defined co-cultures of Geobacter metallireducens and Methanosaeta harundinacea which stoichiometrically converted ethanol to methane. Transcriptomic, radiotracer, and genetic analysis demonstrated that M. harundinacea accepted electrons via DIET for the reduction of carbon dioxide to methane. The discovery that Methanosaeta species, which are abundant in a wide diversity of methanogenic environments, are capable of DIET has important implications not only for the functioning of anaerobic digesters, but also for global methane production.
关键词Rice Field Soil Geobacter-sulfurreducens Acetate Rna Microorganisms Bacteria Hydrogen Formate Identification Association
学科领域Chemistry, Multidisciplinary ; Energy & Fuels ; Engineering, Chemical ; Environmental Sciences
项目资助者Office of Science (BER), U.S. Department of Energy [DE-SC0004485]
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收录类别SCI
语种英语
资助项目海岸带生物学与生物资源利用所重点实验室
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7026
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@microbio.umass.edu
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Rotaru, Amelia-Elena,Shrestha, Pravin Malta,Liu, Fanghua,et al. A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J]. ENERGY & ENVIRONMENTAL SCIENCE,2014,7(1):408-415.
APA Rotaru, Amelia-Elena.,Shrestha, Pravin Malta.,Liu, Fanghua.,Shrestha, Minita.,Shrestha, Devesh.,...&Rotaru, AE .(2014).A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane.ENERGY & ENVIRONMENTAL SCIENCE,7(1),408-415.
MLA Rotaru, Amelia-Elena,et al."A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane".ENERGY & ENVIRONMENTAL SCIENCE 7.1(2014):408-415.
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