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A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane
作者: Rotaru, Amelia-Elena ; Shrestha, Pravin Malta ; Fanghua Liu(刘芳华) ; Shrestha, Minita ; Shrestha, Devesh ; Embree, Mallory ; Zengler, Karsten ; Wardman, Colin ; Nevin, Kelly P. ; Lovley, Derek R.
刊名: ENERGY & ENVIRONMENTAL SCIENCE
ISSN号: 1754-5692
出版日期: 2014-01
卷号: 7, 期号:1, 页码:408-415
关键词: RICE FIELD SOIL ; GEOBACTER-SULFURREDUCENS ; ACETATE ; RNA ; MICROORGANISMS ; BACTERIA ; HYDROGEN ; FORMATE ; IDENTIFICATION ; ASSOCIATION
学科分类: Chemistry, Multidisciplinary ; Energy & Fuels ; Engineering, Chemical ; Environmental Sciences
产权排序: [Rotaru, Amelia-Elena; Shrestha, Pravin Malta; Liu, Fanghua; Shrestha, Minita; Shrestha, Devesh; Wardman, Colin; Nevin, Kelly P.; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA; [Liu, Fanghua] Yantai Inst Coastal Res, Yantai, Peoples R China; [Embree, Mallory; Zengler, Karsten] Univ Calif San Diego, La Jolla, CA 92093 USA
通讯作者: Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@microbio.umass.edu
中文摘要: Anaerobic conversion of organic wastes and biomass to methane is an important bioenergy strategy, which depends on poorly understood mechanisms of interspecies electron transfer to methanogenic microorganisms. Metatranscriptomic analysis of methanogenic aggregates from a brewery wastewater digester, coupled with fluorescence in situ hybridization with specific 16S rRNA probes, revealed that Methanosaeta species were the most abundant and metabolically active methanogens. Methanogens known to reduce carbon dioxide with H2 or formate as the electron donor were rare. Although Methanosaeta have previously been thought to be restricted to acetate as a substrate for methane production, Methanosaeta in the aggregates had a complete complement of genes for the enzymes necessary for the reduction of carbon to methane, and transcript abundance for these genes was high. Furthermore, Geobacter species, the most abundant bacteria in the aggregates, highly expressed genes for ethanol metabolism and for extracellular electron transfer via electrically conductive pili, suggesting that Geobacter and Methanosaeta species were exchanging electrons via direct interspecies electron transfer (DIET). This possibility was further investigated in defined co-cultures of Geobacter metallireducens and Methanosaeta harundinacea which stoichiometrically converted ethanol to methane. Transcriptomic, radiotracer, and genetic analysis demonstrated that M. harundinacea accepted electrons via DIET for the reduction of carbon dioxide to methane. The discovery that Methanosaeta species, which are abundant in a wide diversity of methanogenic environments, are capable of DIET has important implications not only for the functioning of anaerobic digesters, but also for global methane production.
收录类别: SCI
原文出处: 查看原文
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/7026
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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Recommended Citation:
Rotaru, Amelia-Elena,Shrestha, Pravin Malta,Liu, Fanghua,et al. A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J]. ENERGY & ENVIRONMENTAL SCIENCE,2014,7(1):408-415.
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