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Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases
Chen, Lingxin1; Fu, Xiuli1,2; Li, Jinhua1; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
2013
Source PublicationNANOSCALE
ISSN2040-3364
Volume5Issue:13Pages:5905-5911
Contribution Rank[Chen, Lingxin; Fu, Xiuli; Li, Jinhua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China; [Fu, Xiuli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
AbstractIn this work, a simple and sensitive surface-enhanced Raman scattering (SERS) strategy was developed for recognition and detection of trypsin, by using anti-aggregation of 4-mercaptopyridine (4-MPY)-functionalized silver nanoparticles (AgNPs) based on the interaction between protamine and trypsin. The polycationic protamine not only served as a substrate for enzyme hydrolysis but also worked as a medium for SERS enhancement, which could bind negatively charged 4-MPY-functionalized AgNPs and induce their aggregation. The hydrolysis catalyzed with trypsin in sample solution decreased the concentration of free protamine, resulting in the dispersion of AgNPs and thus decreasing the Raman intensity of 4-MPY, by which the trypsin could be sensed optically. A detection level down to 0.1 ng mL(-1) for trypsin was obtained. The induced accumulation of AgNPs modified with Raman reporter 4-MPY largely enhanced the SERS responses. A good linearity was found within the wide range over five orders of magnitude and reasonable relative standard deviations (between 2.4 and 11.6%) were attained. By using trypsin as a model, the new concept can provide an excellent platform for ultrasensitive SERS measurements of various proteases/enzymes which can lead to nanoparticles stability change through catalyzed hydrolysis toward substrate.; In this work, a simple and sensitive surface-enhanced Raman scattering (SERS) strategy was developed for recognition and detection of trypsin, by using anti-aggregation of 4-mercaptopyridine (4-MPY)-functionalized silver nanoparticles (AgNPs) based on the interaction between protamine and trypsin. The polycationic protamine not only served as a substrate for enzyme hydrolysis but also worked as a medium for SERS enhancement, which could bind negatively charged 4-MPY-functionalized AgNPs and induce their aggregation. The hydrolysis catalyzed with trypsin in sample solution decreased the concentration of free protamine, resulting in the dispersion of AgNPs and thus decreasing the Raman intensity of 4-MPY, by which the trypsin could be sensed optically. A detection level down to 0.1 ng mL(-1) for trypsin was obtained. The induced accumulation of AgNPs modified with Raman reporter 4-MPY largely enhanced the SERS responses. A good linearity was found within the wide range over five orders of magnitude and reasonable relative standard deviations (between 2.4 and 11.6%) were attained. By using trypsin as a model, the new concept can provide an excellent platform for ultrasensitive SERS measurements of various proteases/enzymes which can lead to nanoparticles stability change through catalyzed hydrolysis toward substrate.
KeywordLabel-free Gold Nanoparticles Conjugated Polyelectrolyte Graphene Oxide Sers Assay Dyes Spectroscopy Sensitivity Adsorption
Subject AreaChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
Funding OrganizationNational Natural Science Foundation of China [21275158]; Innovation Projects of the Chinese Academy of Sciences [KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences
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Indexed BySCI
WOS KeywordLABEL-FREE ; GOLD NANOPARTICLES ; CONJUGATED POLYELECTROLYTE ; GRAPHENE OXIDE ; SERS ; ASSAY ; DYES ; SPECTROSCOPY ; SENSITIVITY ; ADSORPTION
SubtypeArticle
Language英语
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS IDWOS:000320398300031
Citation statistics
Cited Times:55[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6996
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorChen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Lingxin,Fu, Xiuli,Li, Jinhua,et al. Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases[J]. NANOSCALE,2013,5(13):5905-5911.
APA Chen, Lingxin,Fu, Xiuli,Li, Jinhua,&Chen, LX .(2013).Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases.NANOSCALE,5(13),5905-5911.
MLA Chen, Lingxin,et al."Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases".NANOSCALE 5.13(2013):5905-5911.
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