Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases | |
Chen, Lingxin1; Fu, Xiuli1,2; Li, Jinhua1; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China. lxchen@yic.ac.cn | |
发表期刊 | NANOSCALE |
ISSN | 2040-3364 |
2013 | |
卷号 | 5期号:13页码:5905-5911 |
关键词 | Label-free Gold Nanoparticles Conjugated Polyelectrolyte Graphene Oxide Sers Assay Dyes Spectroscopy Sensitivity Adsorption |
产权排序 | [Chen, Lingxin; Fu, Xiuli; Li, Jinhua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China; [Fu, Xiuli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
英文摘要 | In this work, a simple and sensitive surface-enhanced Raman scattering (SERS) strategy was developed for recognition and detection of trypsin, by using anti-aggregation of 4-mercaptopyridine (4-MPY)-functionalized silver nanoparticles (AgNPs) based on the interaction between protamine and trypsin. The polycationic protamine not only served as a substrate for enzyme hydrolysis but also worked as a medium for SERS enhancement, which could bind negatively charged 4-MPY-functionalized AgNPs and induce their aggregation. The hydrolysis catalyzed with trypsin in sample solution decreased the concentration of free protamine, resulting in the dispersion of AgNPs and thus decreasing the Raman intensity of 4-MPY, by which the trypsin could be sensed optically. A detection level down to 0.1 ng mL(-1) for trypsin was obtained. The induced accumulation of AgNPs modified with Raman reporter 4-MPY largely enhanced the SERS responses. A good linearity was found within the wide range over five orders of magnitude and reasonable relative standard deviations (between 2.4 and 11.6%) were attained. By using trypsin as a model, the new concept can provide an excellent platform for ultrasensitive SERS measurements of various proteases/enzymes which can lead to nanoparticles stability change through catalyzed hydrolysis toward substrate.; In this work, a simple and sensitive surface-enhanced Raman scattering (SERS) strategy was developed for recognition and detection of trypsin, by using anti-aggregation of 4-mercaptopyridine (4-MPY)-functionalized silver nanoparticles (AgNPs) based on the interaction between protamine and trypsin. The polycationic protamine not only served as a substrate for enzyme hydrolysis but also worked as a medium for SERS enhancement, which could bind negatively charged 4-MPY-functionalized AgNPs and induce their aggregation. The hydrolysis catalyzed with trypsin in sample solution decreased the concentration of free protamine, resulting in the dispersion of AgNPs and thus decreasing the Raman intensity of 4-MPY, by which the trypsin could be sensed optically. A detection level down to 0.1 ng mL(-1) for trypsin was obtained. The induced accumulation of AgNPs modified with Raman reporter 4-MPY largely enhanced the SERS responses. A good linearity was found within the wide range over five orders of magnitude and reasonable relative standard deviations (between 2.4 and 11.6%) were attained. By using trypsin as a model, the new concept can provide an excellent platform for ultrasensitive SERS measurements of various proteases/enzymes which can lead to nanoparticles stability change through catalyzed hydrolysis toward substrate. |
文章类型 | Article |
资助机构 | National Natural Science Foundation of China [21275158]; Innovation Projects of the Chinese Academy of Sciences [KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | LABEL-FREE ; GOLD NANOPARTICLES ; CONJUGATED POLYELECTROLYTE ; GRAPHENE OXIDE ; SERS ; ASSAY ; DYES ; SPECTROSCOPY ; SENSITIVITY ; ADSORPTION |
研究领域[WOS] | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
WOS记录号 | WOS:000320398300031 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/6996 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Lingxin,Fu, Xiuli,Li, Jinhua,et al. Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases[J]. NANOSCALE,2013,5(13):5905-5911. |
APA | Chen, Lingxin,Fu, Xiuli,Li, Jinhua,&Chen, LX .(2013).Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases.NANOSCALE,5(13),5905-5911. |
MLA | Chen, Lingxin,et al."Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases".NANOSCALE 5.13(2013):5905-5911. |
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