Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water | |
Zhang, Qianyu1,2; Ding, Jiawang1 ; Kou, Lijuan1,2; Qin, Wei1; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. qyzhang@yic.ac.cn; jwding@yic.ac.cn; ljkou@yic.ac.cn; wqin@yic.ac.cn
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| 发表期刊 | SENSORS
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| ISSN | 1424-8220 |
| 2013-06-01 | |
| 卷号 | 13期号:6页码:6936-6945 |
| 关键词 | Potentiometry Flow Biosensors Ion-selective Electrodes Toxicity Nitrosomonas Europaea |
| 产权排序 | [Zhang, Qianyu; Ding, Jiawang; Kou, Lijuan; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Qianyu; Kou, Lijuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 英文摘要 | A flow biosensor for the detection of toxicity in water using the ammonia-oxidizing bacterium (AOB) Nitrosomonas europaea as a bioreceptor and a polymeric membrane ammonium-selective electrode as a transducer is described. The system is based on the inhibition effects of toxicants on the activity of AOB, which can be evaluated by measuring the ammonium consumption rates with the ammonium-selective membrane electrode. The AOB cells are immobilized on polyethersulfone membranes packed in a holder, while the membrane electrode is placed downstream in the flow cell. Two specific inhibitors of the ammonia oxidation. allylthiourea and thioacetamide. have been tested. The IC50 values defined as the concentration of an inhibitor causing a 50% reduction in the ammonia oxidation activity have been measured as 0.17 mu M and 0.46 mu M for allylthiourea and thioacetamide, respectively. The proposed sensor offers advantages of simplicity, speed and high sensitivity for measuring toxicity in water.; A flow biosensor for the detection of toxicity in water using the ammonia-oxidizing bacterium (AOB) Nitrosomonas europaea as a bioreceptor and a polymeric membrane ammonium-selective electrode as a transducer is described. The system is based on the inhibition effects of toxicants on the activity of AOB, which can be evaluated by measuring the ammonium consumption rates with the ammonium-selective membrane electrode. The AOB cells are immobilized on polyethersulfone membranes packed in a holder, while the membrane electrode is placed downstream in the flow cell. Two specific inhibitors of the ammonia oxidation. allylthiourea and thioacetamide. have been tested. The IC50 values defined as the concentration of an inhibitor causing a 50% reduction in the ammonia oxidation activity have been measured as 0.17 mu M and 0.46 mu M for allylthiourea and thioacetamide, respectively. The proposed sensor offers advantages of simplicity, speed and high sensitivity for measuring toxicity in water. |
| 文章类型 | Article |
| 资助机构 | Instrument Developing Project of the Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081, 21207156]; Taishan Scholar Program of Shandong Province [TS20081159] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | NITROSOMONAS-EUROPAEA ; NITRIFICATION ; INHIBITORS ; BIOASSAY |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000320767600004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6986 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. qyzhang@yic.ac.cn; jwding@yic.ac.cn; ljkou@yic.ac.cn; wqin@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qianyu,Ding, Jiawang,Kou, Lijuan,et al. A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water[J]. SENSORS,2013,13(6):6936-6945. |
| APA | Zhang, Qianyu.,Ding, Jiawang.,Kou, Lijuan.,Qin, Wei.,Qin, W .,...&wqin@yic.ac.cn.(2013).A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water.SENSORS,13(6),6936-6945. |
| MLA | Zhang, Qianyu,et al."A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water".SENSORS 13.6(2013):6936-6945. |
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