Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum | |
Mu, Changkao1; Chen, Leilei1,2,3; Zhao, Jianmin2,3 ; Wang, Chunlin1
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| 发表期刊 | MOLECULAR BIOLOGY REPORTS
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| ISSN | 0301-4851 |
| 2014 | |
| 卷号 | 41期号:1页码:139-144 |
| 关键词 | Venerupis Philippinarum C-type Lectin Gene Expression |
| 产权排序 | [Mu, Changkao; Chen, Leilei; Wang, Chunlin] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Chen, Leilei; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Chen, Leilei; Zhao, Jianmin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Wang, CL (reprint author), Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China. wangchunlin@nbu.edu.cn |
| 英文摘要 | C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97- and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge.; C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97- and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge. |
| 文章类型 | Article |
| 资助机构 | Doctoral Fund of Ministry of Education of China [20103305110003]; National Key Technology Support Program [2011BAD13B0903]; K.C.Wong Magna Fund in Ningbo University |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SCALLOP ARGOPECTEN-IRRADIANS ; SHRIMP LITOPENAEUS-VANNAMEI ; CHLAMYS-FARRERI ; DOMAIN SUPERFAMILY ; RECOGNITION |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000329100500017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6922 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Changkao,Chen, Leilei,Zhao, Jianmin,et al. Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum[J]. MOLECULAR BIOLOGY REPORTS,2014,41(1):139-144. |
| APA | Mu, Changkao,Chen, Leilei,Zhao, Jianmin,&Wang, Chunlin.(2014).Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum.MOLECULAR BIOLOGY REPORTS,41(1),139-144. |
| MLA | Mu, Changkao,et al."Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum".MOLECULAR BIOLOGY REPORTS 41.1(2014):139-144. |
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