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题名:
Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations
作者: You Liping1,3; Ning Xuanxuan2; Chen Leilei1; Zhang Linbao1; Zhao Jianmin1; Liu Xiaoli1,2; Wu Huifeng1
刊名: CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN号: 0254-4059
出版日期: 2014-03-01
卷号: 32, 期号:2, 页码:334-343
关键词: Mytilus galloprovincialis ; small heat shock protein ; superoxide dismutase (SOD) ; catalase (CAT) ; glutathione peroxidase (GPx) ; cadmium
学科分类: Limnology ; Oceanography
产权排序: [You Liping; Chen Leilei; Zhang Linbao; Zhao Jianmin; Liu Xiaoli; Wu Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China; [Ning Xuanxuan; Liu Xiaoli] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [You Liping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者: Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn
中文摘要: Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.
英文摘要: Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.
研究领域[WOS]: Marine & Freshwater Biology ; Oceanography
关键词[WOS]: INTRACELLULAR REDOX STATE ; ALPHA-CRYSTALLIN DOMAIN ; SMALL STRESS-PROTEINS ; OXIDATIVE STRESS ; METAL ACCUMULATION ; PERNA-VIRIDIS ; B-CRYSTALLIN ; CELL-DEATH ; HSP27 ; GLUTATHIONE
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000332739600009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/6897
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China
2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
You Liping,Ning Xuanxuan,Chen Leilei,et al. Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):334-343.
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