Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations
You Liping1,3; Ning Xuanxuan2; Chen Leilei1; Zhang Linbao1; Zhao Jianmin1; Liu Xiaoli1,2; Wu Huifeng1
2014-03-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
卷号32期号:2页码:334-343
产权排序[You Liping; Chen Leilei; Zhang Linbao; Zhao Jianmin; Liu Xiaoli; Wu Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China; [Ning Xuanxuan; Liu Xiaoli] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [You Liping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
摘要Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.; Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.
通讯作者Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn
关键词Mytilus Galloprovincialis Small Heat Shock Protein Superoxide Dismutase (Sod) Catalase (Cat) Glutathione Peroxidase (Gpx) Cadmium
学科领域Limnology ; Oceanography
项目资助者Chinese Academy of Sciences; National Natural Science Foundation of China [41206105]; Key Deployment Program of Chinese Academy of Sciences [KZZD-EW-14-03]
URL查看原文
收录类别SCI
关键词[WOS]INTRACELLULAR REDOX STATE ; ALPHA-CRYSTALLIN DOMAIN ; SMALL STRESS-PROTEINS ; OXIDATIVE STRESS ; METAL ACCUMULATION ; PERNA-VIRIDIS ; B-CRYSTALLIN ; CELL-DEATH ; HSP27 ; GLUTATHIONE
文章类型Article
语种英语
资助项目中国科学院海岸带环境过程与生态修复重点实验室
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS记录号WOS:000332739600009
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6897
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China
2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
推荐引用方式
GB/T 7714
You Liping,Ning Xuanxuan,Chen Leilei,et al. Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):334-343.
APA You Liping.,Ning Xuanxuan.,Chen Leilei.,Zhang Linbao.,Zhao Jianmin.,...&Wu Huifeng.(2014).Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),334-343.
MLA You Liping,et al."Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):334-343.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Expression profiles (1127KB) 开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[You Liping]的文章
[Ning Xuanxuan]的文章
[Chen Leilei]的文章
百度学术
百度学术中相似的文章
[You Liping]的文章
[Ning Xuanxuan]的文章
[Chen Leilei]的文章
必应学术
必应学术中相似的文章
[You Liping]的文章
[Ning Xuanxuan]的文章
[Chen Leilei]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。