YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations
You Liping1,3; Ning Xuanxuan2; Chen Leilei1; Zhang Linbao1; Zhao Jianmin1; Liu Xiaoli1,2; Wu Huifeng1
2014-03-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume32Issue:2Pages:334-343
Contribution Rank[You Liping; Chen Leilei; Zhang Linbao; Zhao Jianmin; Liu Xiaoli; Wu Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China; [Ning Xuanxuan; Liu Xiaoli] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [You Liping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
AbstractSmall heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.; Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.
Corresponding AuthorZhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn
KeywordMytilus Galloprovincialis Small Heat Shock Protein Superoxide Dismutase (Sod) Catalase (Cat) Glutathione Peroxidase (Gpx) Cadmium
Subject AreaLimnology ; Oceanography
Funding OrganizationChinese Academy of Sciences; National Natural Science Foundation of China [41206105]; Key Deployment Program of Chinese Academy of Sciences [KZZD-EW-14-03]
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Indexed BySCI
WOS KeywordINTRACELLULAR REDOX STATE ; ALPHA-CRYSTALLIN DOMAIN ; SMALL STRESS-PROTEINS ; OXIDATIVE STRESS ; METAL ACCUMULATION ; PERNA-VIRIDIS ; B-CRYSTALLIN ; CELL-DEATH ; HSP27 ; GLUTATHIONE
SubtypeArticle
Language英语
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS IDWOS:000332739600009
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6897
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China
2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
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GB/T 7714
You Liping,Ning Xuanxuan,Chen Leilei,et al. Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):334-343.
APA You Liping.,Ning Xuanxuan.,Chen Leilei.,Zhang Linbao.,Zhao Jianmin.,...&Wu Huifeng.(2014).Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),334-343.
MLA You Liping,et al."Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):334-343.
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