Objective To preliminarily isolate cytolytic toxins from Cereus sinensis Verrill and investigate the factors influencing their cytolytic activity. Methods Crude venom from sea anemone was successively obtained by freeze-thaw, homogenate,water extraction and centrifugation. Five parts were isolated from the 60%-90% fraction by ammonium su/fate precipitation and Sephadex G-100 size-exclusion chroma- tograph. The first section （CCT） was shown to be the most potential one by measuring cytolytic activi- ty of them; CU50 ,the best incubation temperature and the best pH of CCT were detected, then the in- fluence of EDTA and metal cations to CCT were investigated. Result CCT was confirmed to be the most potential part by ammonium sulfate precipitation and Sephadex G-100 size-exclusion chromatograph. The CU50 of CCT was 7. 76μg mL-1. the best incubation temperature of CU50 was 40℃ and the best pH of CCT was 8; in vitro cytolytic study of CCT showed that the its cytolytic activity was influenced by EDTA and metal cations mostly. Conclusion The active fraction from Cereus sinensis Verrill was pre- liminarily isolated by ammonium sulfate precipitation and Sephadex G-100 size-exclusion chromato- graph, and the factors influencing cytolytic activity of CCT were detected. This study provides support to discover purified compound subsequently
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