YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas
Cong, Ming1; Wu, Huifeng1; Liu, Xiaoli1,2; Zhao, Jianmin1; Wang, Xuan3; Lv, Jiasen4; Hou, Lin4
Source PublicationECOTOXICOLOGY
ISSN0963-9292
2012-10-01
Volume21Issue:7Pages:1928-1936
KeywordCrassostrea Gigas Metallothionein Cadmium Zinc Antioxidant Enzyme
Contribution Rank[Cong, Ming; Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Liu, Xiaoli] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Wang, Xuan] Ocean Univ China, Qingdao 266100, Shandong, Peoples R China; [Lv, Jiasen; Hou, Lin] Yantai Univ, Ocean Sch, Yantai 264005, Shandong, Peoples R China
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China.
Department中科院海岸带环境过程与生态修复重点实验室
AbstractSequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem beta domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissue-specific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms.; Sequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem beta domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissue-specific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordMESSENGER-RNA EXPRESSION ; PACIFIC OYSTER ; CADMIUM EXPOSURE ; RUDITAPES-DECUSSATUS ; SUPEROXIDE DISMUTASE ; LIPID-PEROXIDATION ; VIRGINICA GMELIN ; STRESS-PROTEINS ; IN-VITRO ; COPPER
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
Funding ProjectCong, Ming; Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Wang, Xuan; Lv, Jiasen; Hou, Lin
WOS IDWOS:000308235500016
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5984
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Ocean Univ China, Qingdao 266100, Shandong, Peoples R China
4.Yantai Univ, Ocean Sch, Yantai 264005, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Cong, Ming,Wu, Huifeng,Liu, Xiaoli,et al. Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas[J]. ECOTOXICOLOGY,2012,21(7):1928-1936.
APA Cong, Ming.,Wu, Huifeng.,Liu, Xiaoli.,Zhao, Jianmin.,Wang, Xuan.,...&Hou, Lin.(2012).Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas.ECOTOXICOLOGY,21(7),1928-1936.
MLA Cong, Ming,et al."Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas".ECOTOXICOLOGY 21.7(2012):1928-1936.
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