A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses

	A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses
	Wang, Xuewei 1,2; Wang, Qing 3; Qin, Wei1
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
	2012-10-01
卷号	38 期号:1 页码:145-150
关键词	Ion-selective Electrodes Biosensors Protamine Protease Heparin Moving-part-free
产权排序	[Wang, Xuewei; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Wang, Xuewei] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Wang, Qing] Yantai Univ, Chem & Biol Coll, Yantai 264005, Peoples R China
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China.
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	Traditional potentiometric polyion-sensitive electrodes can only work effectively in samples with vigorous convection fulfilled by magnetic stirrer, electrode rotator, or other moving components. The dependence on complex moving parts prohibits the fabrication of compact, cost-effective, and energy-effective test devices from the commercial point of view. In this paper, a novel potentiometric sensing protocol without using any moving parts has been proposed for polycationic protamine. In contrast to traditional protamine-sensitive electrodes conditioned by discriminated ion (Na+), the proposed electrode is conditioned with primary ion (protamine). Upon a medium exchange from the conditioning solution into an unstirred sample solution without protamine, protamine loaded in the membrane is stripped into the aqueous phase via ion exchange with aqueous sodium ion, thereby inducing a large potential drop. Interestingly, when the sample solution initially contains protamine, the ion-exchange process has been found to be sensitively inhibited by the sample protamine, and thus the potential drop is suppressed, which forms the basis of the moving-part-free potentiometric polyion sensing strategy. Utilizing the digestion ability of protease to protamine, the electrode was employed to determine the activity of trypsin with a detection limit at least one order of magnitude lower than traditional potentiometric methods. The trypsin inhibitor in both buffer and plasma samples was also sensitively detected with the moving-part-free protamine-sensitive electrode. Finally, the ability of the proposed electrode to detect polyanionic heparin was demonstrated. (C) 2012 Elsevier B.V. All rights reserved.; Traditional potentiometric polyion-sensitive electrodes can only work effectively in samples with vigorous convection fulfilled by magnetic stirrer, electrode rotator, or other moving components. The dependence on complex moving parts prohibits the fabrication of compact, cost-effective, and energy-effective test devices from the commercial point of view. In this paper, a novel potentiometric sensing protocol without using any moving parts has been proposed for polycationic protamine. In contrast to traditional protamine-sensitive electrodes conditioned by discriminated ion (Na+), the proposed electrode is conditioned with primary ion (protamine). Upon a medium exchange from the conditioning solution into an unstirred sample solution without protamine, protamine loaded in the membrane is stripped into the aqueous phase via ion exchange with aqueous sodium ion, thereby inducing a large potential drop. Interestingly, when the sample solution initially contains protamine, the ion-exchange process has been found to be sensitively inhibited by the sample protamine, and thus the potential drop is suppressed, which forms the basis of the moving-part-free potentiometric polyion sensing strategy. Utilizing the digestion ability of protease to protamine, the electrode was employed to determine the activity of trypsin with a detection limit at least one order of magnitude lower than traditional potentiometric methods. The trypsin inhibitor in both buffer and plasma samples was also sensitively detected with the moving-part-free protamine-sensitive electrode. Finally, the ability of the proposed electrode to detect polyanionic heparin was demonstrated. (C) 2012 Elsevier B.V. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of China [41176081]; Taishan Scholar Program of Shandong Province [TS20081159]
收录类别	SCI
语种	英语
关键词[WOS]	POTENTIOMETRIC POLYION SENSORS ; ELECTROCHEMICAL ASSAY ; LABEL-FREE ; BIOANALYTICAL APPLICATIONS ; PEPTIDASE ACTIVITIES ; CARDIAC-SURGERY ; HEPARIN ; BINDING ; APROTININ ; MECHANISM
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS记录号	WOS:000308455700023
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/5950
专题	中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Yantai Univ, Chem & Biol Coll, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuewei,Wang, Qing,Qin, Wei. A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses[J]. BIOSENSORS & BIOELECTRONICS,2012,38(1):145-150.
APA	Wang, Xuewei,Wang, Qing,&Qin, Wei.(2012).A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses.BIOSENSORS & BIOELECTRONICS,38(1),145-150.
MLA	Wang, Xuewei,et al."A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses".BIOSENSORS & BIOELECTRONICS 38.1(2012):145-150.

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