| Identification of glutaredoxin 1 and glutaredoxin 2 genes from Venerupis philippinarum and their responses to benzo[a]pyrene and bacterial challenge | |
Mu, Changkao2; Wang, Qing1 ; Yuan, Zeyi3; Zhang, Zhendong4; Wang, Chunlin2
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2012-03-01 | |
| 卷号 | 32期号:3页码:482-488 |
| 关键词 | Glutaredoxin Venerupis Philippinarum Benzo[a]Pyrene Exposure Bacterial Challenge |
| 产权排序 | [Wang, Qing] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Mu, Changkao; Wang, Chunlin] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Yuan, Zeyi] Natl Marine Data & Informat Serv, Tianjin 300171, Peoples R China; [Zhang, Zhendong] Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China |
| 通讯作者 | Wang, Q (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. qingwang@yic.ac.cn ; wangchunlin@nbu.edu.cn |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Glutaredoxin (abbreviated as Grx) is an important ubiquitous disulfide reductase, which can protect organisms against oxidative stresses. In the present study, a monothiol glutaredoxin gene (named as VpGrx1) and a dithiol glutaredoxin gene (named as VpGrx2) were identified from Venerupis philippinarum. Similar to most Grx2s, VpGrx2 possessed the conserved catalytic residues (C-P-Y-C) and other conserved features critical for the fundamental structure and function of Grx2s, while the active motif (C-G-Y-S) of VpGrx1 was different from the counterpart in other Gods. Quantitative Real-time PCR assay showed that VpGrx1 and VpGrx2 transcripts were detected in a wide range of tissues and mainly distributed in gills and hepatopancreas. After Vibrio challenge, both the expression levels of VpGrx1 and VoGnc2 mRNA in hemocytes were significantly up-regulated at 24 h. As concerned to benzo[a]pyrene (BaP) exposure, the expression levels of VpGrx1 and VpGrx2 transcripts in hepatopancreas were also significantly induced at 24 h. These results suggested that ROS could be induced through the respiratory burst to clear the invading bacteria and pollutants. VpGrx1 and VpGrx2 perhaps involved in the regulation of redox homeostasis and innate immune responses of V. philippinarum.; Glutaredoxin (abbreviated as Grx) is an important ubiquitous disulfide reductase, which can protect organisms against oxidative stresses. In the present study, a monothiol glutaredoxin gene (named as VpGrx1) and a dithiol glutaredoxin gene (named as VpGrx2) were identified from Venerupis philippinarum. Similar to most Grx2s, VpGrx2 possessed the conserved catalytic residues (C-P-Y-C) and other conserved features critical for the fundamental structure and function of Grx2s, while the active motif (C-G-Y-S) of VpGrx1 was different from the counterpart in other Gods. Quantitative Real-time PCR assay showed that VpGrx1 and VpGrx2 transcripts were detected in a wide range of tissues and mainly distributed in gills and hepatopancreas. After Vibrio challenge, both the expression levels of VpGrx1 and VoGnc2 mRNA in hemocytes were significantly up-regulated at 24 h. As concerned to benzo[a]pyrene (BaP) exposure, the expression levels of VpGrx1 and VpGrx2 transcripts in hepatopancreas were also significantly induced at 24 h. These results suggested that ROS could be induced through the respiratory burst to clear the invading bacteria and pollutants. VpGrx1 and VpGrx2 perhaps involved in the regulation of redox homeostasis and innate immune responses of V. philippinarum. (C) 2011 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Key Technology Support Program[2011BAD13B00]; K.C. Wong Magna Fund |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | THIOREDOXIN ; PROTEIN ; EXPRESSION ; SYSTEMS ; CLONING ; MITOCHONDRIAL ; ISOFORMS ; CADMIUM ; STRESS ; GROWTH |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000300817300013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5611 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 3.Natl Marine Data & Informat Serv, Tianjin 300171, Peoples R China 4.Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Changkao,Wang, Qing,Yuan, Zeyi,et al. Identification of glutaredoxin 1 and glutaredoxin 2 genes from Venerupis philippinarum and their responses to benzo[a]pyrene and bacterial challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(3):482-488. |
| APA | Mu, Changkao,Wang, Qing,Yuan, Zeyi,Zhang, Zhendong,&Wang, Chunlin.(2012).Identification of glutaredoxin 1 and glutaredoxin 2 genes from Venerupis philippinarum and their responses to benzo[a]pyrene and bacterial challenge.FISH & SHELLFISH IMMUNOLOGY,32(3),482-488. |
| MLA | Mu, Changkao,et al."Identification of glutaredoxin 1 and glutaredoxin 2 genes from Venerupis philippinarum and their responses to benzo[a]pyrene and bacterial challenge".FISH & SHELLFISH IMMUNOLOGY 32.3(2012):482-488. |
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