| Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions | |
Wang, Xuewei1,2; Ding, Jiawang1 ; Song, Wenjing1,2; Xie, Kang1,3; Qin, Wei1
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| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| ISSN | 0925-4005 |
| 2012-01-03 | |
| 卷号 | 161期号:1页码:1119-1123 |
| 关键词 | Potentiometry Ion-selective Electrode Polyion Protamine Heparin Electrode Conditioning |
| 产权排序 | [Wang, Xuewei; Ding, Jiawang; Song, Wenjing; Xie, Kang; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Xuewei; Song, Wenjing] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xie, Kang] Yantai Univ, Coll Chem & Biol, Yantai 264005, Peoples R China |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Chunhui Rd 17, Yantai 264003, Peoples R China. wqin@yic.ac.cn |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | A novel operation mode for development of sensitive and renewable polymeric membrane polyion-sensitive electrodes has been explored in this paper. In contrast to the traditional polyion sensors which are conditioned with highly discriminated ions, the proposed polycation-sensitive membrane electrode with dinonylnaphthalene sulfonate as the ion-exchanger is conditioned with the primary ion (protamine), and activated with the discriminated ion (Na+) before measurement. Using this protocol, the membrane with protamine in the membrane bulk but with discriminated ions rich at the membrane outer surface can be prepared. The protamine inside the membrane efficiently suppresses the fluxes of protamine from the sample-membrane interface into the membrane bulk, thus decreasing the detection limit toward protamine by one order of magnitude. With the reproducible protamine fluxes obtained by the conditioning and activation steps, the protamine-sensitive electrode could be reused without significant signal deterioration. Titrimetric analysis of heparin with this novel technique has been demonstrated. The proposed polyion sensing platform offers much promise in the detection of polyions as well as in other polyion-involved bioanalyses.; A novel operation mode for development of sensitive and renewable polymeric membrane polyion-sensitive electrodes has been explored in this paper. In contrast to the traditional polyion sensors which are conditioned with highly discriminated ions, the proposed polycation-sensitive membrane electrode with dinonylnaphthalene sulfonate as the ion-exchanger is conditioned with the primary ion (protamine), and activated with the discriminated ion (Na+) before measurement. Using this protocol, the membrane with protamine in the membrane bulk but with discriminated ions rich at the membrane outer surface can be prepared. The protamine inside the membrane efficiently suppresses the fluxes of protamine from the sample-membrane interface into the membrane bulk, thus decreasing the detection limit toward protamine by one order of magnitude. With the reproducible protamine fluxes obtained by the conditioning and activation steps, the protamine-sensitive electrode could be reused without significant signal deterioration. Titrimetric analysis of heparin with this novel technique has been demonstrated. The proposed polyion sensing platform offers much promise in the detection of polyions as well as in other polyion-involved bioanalyses. (c) 2011 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China[41176081]; Outstanding Youth Natural Science Foundation of Shandong Province[JQ200814]; Taishan Scholar Program of Shandong Province[TS20081159] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | RESPONSE CHARACTERISTICS ; ELECTROCHEMICAL ASSAY ; REVERSIBLE DETECTION ; HEPARIN ; PROTAMINE ; SENSORS ; POTENTIOMETRY ; POLYANIONS ; BINDING |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000301549400153 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5531 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Yantai Univ, Coll Chem & Biol, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xuewei,Ding, Jiawang,Song, Wenjing,et al. Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions[J]. SENSORS AND ACTUATORS B-CHEMICAL,2012,161(1):1119-1123. |
| APA | Wang, Xuewei,Ding, Jiawang,Song, Wenjing,Xie, Kang,&Qin, Wei.(2012).Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions.SENSORS AND ACTUATORS B-CHEMICAL,161(1),1119-1123. |
| MLA | Wang, Xuewei,et al."Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions".SENSORS AND ACTUATORS B-CHEMICAL 161.1(2012):1119-1123. |
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