| Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51 | |
Zhang, Wei-Wei1,2,3; Wen, Ying-Ying1,2,3,4; Niu, Zong-Liang1,2,3; Yin, Kun1,2,3,4; Xu, Dong-Xue5; Chen, Ling-Xin1,2,3
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| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| ISSN | 0959-3993 |
| 2012-02-01 | |
| 卷号 | 28期号:2页码:447-452 |
| 关键词 | Sulfonamides Escherichia Sp. Acinetobacter Sp. Degradation Cytotoxicity |
| 产权排序 | [Zhang, Wei-Wei; Wen, Ying-Ying; Niu, Zong-Liang; Yin, Kun; Chen, Ling-Xin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhang, Wei-Wei; Wen, Ying-Ying; Niu, Zong-Liang; Yin, Kun; Chen, Ling-Xin] Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Wei-Wei; Wen, Ying-Ying; Niu, Zong-Liang; Yin, Kun; Chen, Ling-Xin] Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; [Wen, Ying-Ying; Yin, Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xu, Dong-Xue] China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | With the intensive application of sulfonamides in aquaculture and animal husbandry and the increase of sulfonamides discharged into the environments, there is an increasing need to find a way to remediate sulfonamide-contaminated environments. Two bacterial strains capable of degrading sulfonamides, HS21 and HS51, were isolated from marine environments. HS21 and HS51 were identified as members of Escherichia sp. and Acinetobacter sp., respectively, based on 16S rRNA gene sequencing. Degradation of each sulfonamide by Escherichia sp. HS21 and Acinetobacter sp. HS51 was characterized using capillary electrophoresis. About 66 or 72% of sulfapyridine and 45 or 67% of sulfathiazole contained in the media was degraded by Escherichia sp. HS21 or Acinetobacter sp. HS51, respectively, after incubation for 2 days. The supernatant from culture of Escherichia sp. HS21 or Acinetobacter sp. HS51 grown in sulfapyridine or sulfathiazole contained media had much attenuated cytotoxicity against HeLa cells. These results suggest that Escherichia sp. HS21 and Acinetobacter sp. HS51 are new bacterial resources for biodegrading sulfonamides and indicate the potential of isolated strains for the bioremediation of sulfonamide-polluted environments.; With the intensive application of sulfonamides in aquaculture and animal husbandry and the increase of sulfonamides discharged into the environments, there is an increasing need to find a way to remediate sulfonamide-contaminated environments. Two bacterial strains capable of degrading sulfonamides, HS21 and HS51, were isolated from marine environments. HS21 and HS51 were identified as members of Escherichia sp. and Acinetobacter sp., respectively, based on 16S rRNA gene sequencing. Degradation of each sulfonamide by Escherichia sp. HS21 and Acinetobacter sp. HS51 was characterized using capillary electrophoresis. About 66 or 72% of sulfapyridine and 45 or 67% of sulfathiazole contained in the media was degraded by Escherichia sp. HS21 or Acinetobacter sp. HS51, respectively, after incubation for 2 days. The supernatant from culture of Escherichia sp. HS21 or Acinetobacter sp. HS51 grown in sulfapyridine or sulfathiazole contained media had much attenuated cytotoxicity against HeLa cells. These results suggest that Escherichia sp. HS21 and Acinetobacter sp. HS51 are new bacterial resources for biodegrading sulfonamides and indicate the potential of isolated strains for the bioremediation of sulfonamide-polluted environments. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences[KZCX2-EW-206]; National Natural Science Foundation of China (NSFC)[20975089]; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences[Kf201012]; Department of Science and Technology of Yantai City of China[2010235] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | TANDEM MASS-SPECTROMETRY ; WASTE-WATER CONTAMINANTS ; AQUATIC ENVIRONMENT ; NATIONAL RECONNAISSANCE ; HUMAN PHARMACEUTICALS ; CARBONIC-ANHYDRASE ; UNITED-STATES ; P-TSA ; O-TSA ; ANTIBIOTICS |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000300014300005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5505 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 5.China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei-Wei,Wen, Ying-Ying,Niu, Zong-Liang,et al. Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2012,28(2):447-452. |
| APA | Zhang, Wei-Wei,Wen, Ying-Ying,Niu, Zong-Liang,Yin, Kun,Xu, Dong-Xue,&Chen, Ling-Xin.(2012).Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,28(2),447-452. |
| MLA | Zhang, Wei-Wei,et al."Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 28.2(2012):447-452. |
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