Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine
Zhang, Weiwei1,2,3; Xu, Dongxue4,5; Niu, Zongliang1,2,3; Yin, Kun1,2,3,5; Liu, Ping1,2,3; Chen, Lingxin1,2,3
发表期刊BIODEGRADATION
ISSN0923-9820
2012-06-01
卷号23期号:3页码:431-439
关键词Sulfadoxine Pseudomonas Sp. Degradation Cytotoxicity
产权排序[Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Xu, Dongxue] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Xu, Dongxue; Yin, Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
作者部门环境化学实验室 
英文摘要Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp. based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l(-1) sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30A degrees C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l(-1) and between 2.0 and 8.0 g l(-1), respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxine-contaminated environments.; Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp. based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l(-1) sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30A degrees C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l(-1) and between 2.0 and 8.0 g l(-1), respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxine-contaminated environments.
文章类型Article
资助机构Chinese Academy of Sciences[KZCX2-EW-206]; National Natural Science Foundation of China (NSFC)[20975089]; Doctoral Foundation of Shandong Province[BS2011SW056]; Department of Science and Technology of Yantai City of China[2010235]; Chinese Academy of Sciences
收录类别SCI
语种英语
关键词[WOS]TANDEM MASS-SPECTROMETRY ; WASTE-WATER CONTAMINANTS ; SULFONAMIDES P-TSA ; FALCIPARUM-MALARIA ; NATIONAL RECONNAISSANCE ; HUMAN PHARMACEUTICALS ; AQUATIC ENVIRONMENT ; UNITED-STATES ; O-TSA ; BIODEGRADATION
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000303377300009
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5503
专题中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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GB/T 7714
Zhang, Weiwei,Xu, Dongxue,Niu, Zongliang,et al. Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine[J]. BIODEGRADATION,2012,23(3):431-439.
APA Zhang, Weiwei,Xu, Dongxue,Niu, Zongliang,Yin, Kun,Liu, Ping,&Chen, Lingxin.(2012).Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine.BIODEGRADATION,23(3),431-439.
MLA Zhang, Weiwei,et al."Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine".BIODEGRADATION 23.3(2012):431-439.
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