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Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine
Chen, Yan1,2; Ding, Jiawang1; Qin, Wei1
2012
发表期刊ANALYST
卷号137期号:8页码:1944-1949
产权排序[Chen, Yan; Ding, Jiawang; Qin, Wei] CAS, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Chen, Yan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
摘要A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of heparin. The incorporation of tetradodecylammonium tetrakis(4-chlorophenyl)borate as a lipophilic salt in the membrane dramatically improves the sensor's selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane, and a stable potential baseline can be obtained in the presence of an electrolyte background. The electrostatic binding interaction between heparin and protamine decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions, thus decreasing the membrane potential. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear relationship between the initial slope of the potential change and the heparin concentration in the range of 0.025-1.25 U mL(-1) with an improved detection limit of 0.01 U mL(-1).; A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of heparin. The incorporation of tetradodecylammonium tetrakis(4-chlorophenyl)borate as a lipophilic salt in the membrane dramatically improves the sensor's selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane, and a stable potential baseline can be obtained in the presence of an electrolyte background. The electrostatic binding interaction between heparin and protamine decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions, thus decreasing the membrane potential. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear relationship between the initial slope of the potential change and the heparin concentration in the range of 0.025-1.25 U mL(-1) with an improved detection limit of 0.01 U mL(-1).
关键词Potentiometric Polyanion Sensors Bioanalytical Applications Electrochemical Sensor Selective Electrodes Response Mechanism Polyion Contaminants Binding Assay
学科领域Chemistry
项目资助者National Natural Science Foundation of China[41176081]; Outstanding Youth Natural Science Foundation of Shandong Province[JQ200814]; Taishan Scholar Program of Shandong Province[TS20081159]
收录类别SCI
关键词[WOS]POTENTIOMETRIC POLYANION SENSORS ; BIOANALYTICAL APPLICATIONS ; ELECTROCHEMICAL SENSOR ; SELECTIVE ELECTRODES ; RESPONSE MECHANISM ; POLYION ; CONTAMINANTS ; BINDING ; ASSAY
文章类型Article
语种英语
WOS研究方向Chemistry
WOS记录号WOS:000301674600030
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5412
专题环境化学实验室
作者单位1.CAS, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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GB/T 7714
Chen, Yan,Ding, Jiawang,Qin, Wei. Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine[J]. ANALYST,2012,137(8):1944-1949.
APA Chen, Yan,Ding, Jiawang,&Qin, Wei.(2012).Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine.ANALYST,137(8),1944-1949.
MLA Chen, Yan,et al."Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine".ANALYST 137.8(2012):1944-1949.
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