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题名:
Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine
作者: Chen, Yan1,2; Ding, JiaWang(丁家旺)1; Wei Qin(秦伟)1
刊名: ANALYST
出版日期: 2012
卷号: 137, 期号:8, 页码:1944-1949
关键词: POTENTIOMETRIC POLYANION SENSORS ; BIOANALYTICAL APPLICATIONS ; ELECTROCHEMICAL SENSOR ; SELECTIVE ELECTRODES ; RESPONSE MECHANISM ; POLYION ; CONTAMINANTS ; BINDING ; ASSAY
学科分类: Chemistry
产权排序: [Chen, Yan; Ding, Jiawang; Qin, Wei] CAS, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Chen, Yan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
中文摘要: A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of heparin. The incorporation of tetradodecylammonium tetrakis(4-chlorophenyl)borate as a lipophilic salt in the membrane dramatically improves the sensor's selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane, and a stable potential baseline can be obtained in the presence of an electrolyte background. The electrostatic binding interaction between heparin and protamine decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions, thus decreasing the membrane potential. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear relationship between the initial slope of the potential change and the heparin concentration in the range of 0.025-1.25 U mL(-1) with an improved detection limit of 0.01 U mL(-1).
英文摘要: A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of heparin. The incorporation of tetradodecylammonium tetrakis(4-chlorophenyl)borate as a lipophilic salt in the membrane dramatically improves the sensor's selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane, and a stable potential baseline can be obtained in the presence of an electrolyte background. The electrostatic binding interaction between heparin and protamine decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions, thus decreasing the membrane potential. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear relationship between the initial slope of the potential change and the heparin concentration in the range of 0.025-1.25 U mL(-1) with an improved detection limit of 0.01 U mL(-1).
研究领域[WOS]: Chemistry
关键词[WOS]: POTENTIOMETRIC POLYANION SENSORS ; BIOANALYTICAL APPLICATIONS ; ELECTROCHEMICAL SENSOR ; SELECTIVE ELECTRODES ; RESPONSE MECHANISM ; POLYION ; CONTAMINANTS ; BINDING ; ASSAY
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000301674600030
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/5412
Appears in Collections:环境化学实验室_期刊论文

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作者单位: 1.CAS, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Chen, Yan,Ding, Jiawang,Qin, Wei. Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine[J]. ANALYST,2012,137(8):1944-1949.
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