Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels | |
Li, Jinhua1![]() ![]() | |
发表期刊 | JOURNAL OF MATERIALS CHEMISTRY
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ISSN | 0959-9428 |
2011 | |
卷号 | 21期号:48页码:19267-19274 |
关键词 | Solid-phase Extraction Polymers Fluorescence Nanosensor Biosensor Impedance Samples Sensor |
产权排序 | [Li, Jinhua; Zhang, Zhong; Xu, Shoufang; Chen, Lingxin; Zhou, Na] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China; [Zhang, Zhong; Xiong, Hua; Peng, Hailong] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China; [Xu, Shoufang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China |
作者部门 | 环境化学实验室 |
英文摘要 | A novel colorimetric sensor for cholesterol assay was constructed by combining a molecular imprinting technique with photonic crystals. The molecularly imprinted photonic hydrogel (MIPH) film was prepared by a non-covalent, self-assembly approach using cholesterol as a template molecule, and exhibited a highly ordered three-dimensional macroporous structure characterized by scanning electron microscopy under the optimized imprinting conditions. Various factors affecting rebinding of cholesterol are discussed along with recognition specificity studies on its analogues of stigmasterol and ergosterol through estimation of UV-Vis and electrochemical impedance spectroscopy. The MIPH film generated a significantly readable optical signal directly self-reporting within less than 2 min upon binding cholesterol. The colorimetric measurement of cholesterol concentration strongly relies on the fact that the blue shift effect of the Bragg diffraction peak of the MIPH is gradually enlarged with the increase of cholesterol amounts. The detection level approached 10(-13) g mL(-1), which is comparable to that of fluorescence measurements. The simultaneous possession of high selectivity, high sensitivity, high stability, easy operation and being label-free enables this sensor to be potentially applicable for rapid on-site detection of trace cholesterol.; A novel colorimetric sensor for cholesterol assay was constructed by combining a molecular imprinting technique with photonic crystals. The molecularly imprinted photonic hydrogel (MIPH) film was prepared by a non-covalent, self-assembly approach using cholesterol as a template molecule, and exhibited a highly ordered three-dimensional macroporous structure characterized by scanning electron microscopy under the optimized imprinting conditions. Various factors affecting rebinding of cholesterol are discussed along with recognition specificity studies on its analogues of stigmasterol and ergosterol through estimation of UV-Vis and electrochemical impedance spectroscopy. The MIPH film generated a significantly readable optical signal directly self-reporting within less than 2 min upon binding cholesterol. The colorimetric measurement of cholesterol concentration strongly relies on the fact that the blue shift effect of the Bragg diffraction peak of the MIPH is gradually enlarged with the increase of cholesterol amounts. The detection level approached 10(-13) g mL(-1), which is comparable to that of fluorescence measurements. The simultaneous possession of high selectivity, high sensitivity, high stability, easy operation and being label-free enables this sensor to be potentially applicable for rapid on-site detection of trace cholesterol. |
文章类型 | Article |
资助机构 | National Natural Science Foundation of China[20975089, 21105117, 31160317]; Chinese Academy of Sciences[KZCX2-EW-206]; Department of Science and Technology of Shandong Province/Yantai City of China[2008GG20005005/2010158]; Natural Science Foundation of Shandong Province of China[ZR2010BQ027]; State Key Laboratory of Food Science and Technology of Nanchang University of China[SKLF-KF-201006, SKLF-MB-201005]; Department of Science and Technology of Jiangxi Province of China[Ganke-fa2010J217] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | SOLID-PHASE EXTRACTION ; POLYMERS ; FLUORESCENCE ; NANOSENSOR ; BIOSENSOR ; IMPEDANCE ; SAMPLES ; SENSOR |
研究领域[WOS] | Chemistry ; Materials Science |
WOS记录号 | WOS:000297559400012 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/5340 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China 2.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Jinhua,Zhang, Zhong,Xu, Shoufang,et al. Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels[J]. JOURNAL OF MATERIALS CHEMISTRY,2011,21(48):19267-19274. |
APA | Li, Jinhua.,Zhang, Zhong.,Xu, Shoufang.,Chen, Lingxin.,Zhou, Na.,...&Peng, Hailong.(2011).Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels.JOURNAL OF MATERIALS CHEMISTRY,21(48),19267-19274. |
MLA | Li, Jinhua,et al."Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels".JOURNAL OF MATERIALS CHEMISTRY 21.48(2011):19267-19274. |
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