Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels
Li, Jinhua1; Zhang, Zhong1,2; Xu, Shoufang1,3; Chen, Lingxin1; Zhou, Na1; Xiong, Hua2; Peng, Hailong2
发表期刊JOURNAL OF MATERIALS CHEMISTRY
ISSN0959-9428
2011
卷号21期号:48页码:19267-19274
关键词Solid-phase Extraction Polymers Fluorescence Nanosensor Biosensor Impedance Samples Sensor
产权排序[Li, Jinhua; Zhang, Zhong; Xu, Shoufang; Chen, Lingxin; Zhou, Na] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China; [Zhang, Zhong; Xiong, Hua; Peng, Hailong] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China; [Xu, Shoufang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China
作者部门环境化学实验室 
英文摘要A novel colorimetric sensor for cholesterol assay was constructed by combining a molecular imprinting technique with photonic crystals. The molecularly imprinted photonic hydrogel (MIPH) film was prepared by a non-covalent, self-assembly approach using cholesterol as a template molecule, and exhibited a highly ordered three-dimensional macroporous structure characterized by scanning electron microscopy under the optimized imprinting conditions. Various factors affecting rebinding of cholesterol are discussed along with recognition specificity studies on its analogues of stigmasterol and ergosterol through estimation of UV-Vis and electrochemical impedance spectroscopy. The MIPH film generated a significantly readable optical signal directly self-reporting within less than 2 min upon binding cholesterol. The colorimetric measurement of cholesterol concentration strongly relies on the fact that the blue shift effect of the Bragg diffraction peak of the MIPH is gradually enlarged with the increase of cholesterol amounts. The detection level approached 10(-13) g mL(-1), which is comparable to that of fluorescence measurements. The simultaneous possession of high selectivity, high sensitivity, high stability, easy operation and being label-free enables this sensor to be potentially applicable for rapid on-site detection of trace cholesterol.; A novel colorimetric sensor for cholesterol assay was constructed by combining a molecular imprinting technique with photonic crystals. The molecularly imprinted photonic hydrogel (MIPH) film was prepared by a non-covalent, self-assembly approach using cholesterol as a template molecule, and exhibited a highly ordered three-dimensional macroporous structure characterized by scanning electron microscopy under the optimized imprinting conditions. Various factors affecting rebinding of cholesterol are discussed along with recognition specificity studies on its analogues of stigmasterol and ergosterol through estimation of UV-Vis and electrochemical impedance spectroscopy. The MIPH film generated a significantly readable optical signal directly self-reporting within less than 2 min upon binding cholesterol. The colorimetric measurement of cholesterol concentration strongly relies on the fact that the blue shift effect of the Bragg diffraction peak of the MIPH is gradually enlarged with the increase of cholesterol amounts. The detection level approached 10(-13) g mL(-1), which is comparable to that of fluorescence measurements. The simultaneous possession of high selectivity, high sensitivity, high stability, easy operation and being label-free enables this sensor to be potentially applicable for rapid on-site detection of trace cholesterol.
文章类型Article
资助机构National Natural Science Foundation of China[20975089, 21105117, 31160317]; Chinese Academy of Sciences[KZCX2-EW-206]; Department of Science and Technology of Shandong Province/Yantai City of China[2008GG20005005/2010158]; Natural Science Foundation of Shandong Province of China[ZR2010BQ027]; State Key Laboratory of Food Science and Technology of Nanchang University of China[SKLF-KF-201006, SKLF-MB-201005]; Department of Science and Technology of Jiangxi Province of China[Ganke-fa2010J217]
收录类别SCI
语种英语
关键词[WOS]SOLID-PHASE EXTRACTION ; POLYMERS ; FLUORESCENCE ; NANOSENSOR ; BIOSENSOR ; IMPEDANCE ; SAMPLES ; SENSOR
研究领域[WOS]Chemistry ; Materials Science
WOS记录号WOS:000297559400012
引用统计
被引频次:116[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5340
专题中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China
2.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Li, Jinhua,Zhang, Zhong,Xu, Shoufang,et al. Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels[J]. JOURNAL OF MATERIALS CHEMISTRY,2011,21(48):19267-19274.
APA Li, Jinhua.,Zhang, Zhong.,Xu, Shoufang.,Chen, Lingxin.,Zhou, Na.,...&Peng, Hailong.(2011).Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels.JOURNAL OF MATERIALS CHEMISTRY,21(48),19267-19274.
MLA Li, Jinhua,et al."Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels".JOURNAL OF MATERIALS CHEMISTRY 21.48(2011):19267-19274.
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