| ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY | |
Li, Jinhua1,2 ; Cai, Zongwei2; Xu, Shoufang1,4; Liao, Chunyang1; Song, Xingliang3; Chen, Lingxin1
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| 发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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| ISSN | 1082-6076 |
| 2011 | |
| 卷号 | 34期号:15页码:1578-1593 |
| 关键词 | Electrospray Ionization High Performance Liquid Chromatography Human Urine Mass Spectrometry Porphyrins Solid-phase Extraction |
| 产权排序 | [Li, Jinhua; Xu, Shoufang; Liao, Chunyang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li, Jinhua; Cai, Zongwei] Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China; [Song, Xingliang] Linyi Univ, Dept Chem, Linyi, Peoples R China; [Xu, Shoufang] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.; A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China[20975089, 20907039]; Department of Science and Technology of Shandong Province of China[2008GG20005005, 2010GSF10222]; Natural Science Foundation of Shandong Province of China[Y2007B38]; Yantai Research and Development Program[2007323]; Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; FLUORESCENCE DETECTION ; HPLC METHOD ; SEPARATION ; EXTRACTION ; DIAGNOSIS ; STACKING ; ISOMERS ; CLEANUP |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000296231000009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5326 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China 3.Linyi Univ, Dept Chem, Linyi, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jinhua,Cai, Zongwei,Xu, Shoufang,et al. ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2011,34(15):1578-1593. |
| APA | Li, Jinhua,Cai, Zongwei,Xu, Shoufang,Liao, Chunyang,Song, Xingliang,&Chen, Lingxin.(2011).ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,34(15),1578-1593. |
| MLA | Li, Jinhua,et al."ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 34.15(2011):1578-1593. |
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