| Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure | |
Li, Chenghua1,2,3; Li, Lingyun4; Liu, Feng5; Ning, Xuanxuan6; Chen, Aiqin1,2; Zhang, Linbao1,2; Wu, Huifeng1,2 ; Zhao, Jianmin1,2
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2011 | |
| 卷号 | 30期号:1页码:447-450 |
| 关键词 | Venerupis Philippinarum Hsp40 Vibrio Anguillarum Cu(2+) Cd(2+) |
| 产权排序 | [Li, Chenghua; Chen, Aiqin; Zhang, Linbao; Wu, Huifeng; Zhao, Jianmin] CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Li, Chenghua; Chen, Aiqin; Zhang, Linbao; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Li, Chenghua] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Li, Lingyun] NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China; [Liu, Feng] China Agr Univ Yantai, Yantai 264670, Peoples R China; [Ning, Xuanxuan] Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai 264006, Peoples R China |
| 通讯作者 | Zhao, JM, CAS, Key Lab Coastal Zone Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China.jmzhao@yic.ac.cn |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | HSP40 was an understudied protein family with co-chaperone activity. In the present study, a HSP40 homology was cloned from Venerupis philippinarum haemocytes (designated as VpHSP40) by EST analysis and RACE approaches. The expression profiles of VpHSP40 under Vibrio anguillarum challenge and heavy metal exposure were investigated by quantitative real-time RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression, and the highest expression level was detected at 24 h post-infection with 6.0-fold increase compared with that in the control group. During heavy metal exposure experiment, the expression of VpHSP40 could also be induced by Cu2+ and Cd2+ at different concentration, where Cu2+ displayed more toxic effect on clam than that of Cd2+. Concerning the same heavy metal, varied effect on VpHSP40 expression was detected at different concentration of heavy metal. Taking together, these results suggested that VpHSP40 was perhaps involved in mediating the immune responses and environmental stresses in V. philippinarum. (C) 2010 Elsevier Ltd. All rights reserved.; HSP40 was an understudied protein family with co-chaperone activity. In the present study, a HSP40 homology was cloned from Venerupis philippinarum haemocytes (designated as VpHSP40) by EST analysis and RACE approaches. The expression profiles of VpHSP40 under Vibrio anguillarum challenge and heavy metal exposure were investigated by quantitative real-time RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression, and the highest expression level was detected at 24 h post-infection with 6.0-fold increase compared with that in the control group. During heavy metal exposure experiment, the expression of VpHSP40 could also be induced by Cu(2+) and Cd(2+) at different concentration, where Cu(2+) displayed more toxic effect on clam than that of Cd(2+). Concerning the same heavy metal, varied effect on VpHSP40 expression was detected at different concentration of heavy metal. Taking together, these results suggested that VpHSP40 was perhaps involved in mediating the immune responses and environmental stresses in V. philippinarum. (C) 2010 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MESSENGER-RNA EXPRESSION ; SHOCK-PROTEIN 40 ; MOLECULAR CHAPERONES ; HSP70 GENE ; CELLS ; CLONING ; STRESS |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000286905800058 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4883 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 4.NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China 5.China Agr Univ Yantai, Yantai 264670, Peoples R China 6.Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chenghua,Li, Lingyun,Liu, Feng,et al. Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):447-450. |
| APA | Li, Chenghua.,Li, Lingyun.,Liu, Feng.,Ning, Xuanxuan.,Chen, Aiqin.,...&Zhao, Jianmin.(2011).Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure.FISH & SHELLFISH IMMUNOLOGY,30(1),447-450. |
| MLA | Li, Chenghua,et al."Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):447-450. |
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