| Response of phytoplankton to nitrogen addition in the Taiwan strait upwelling region: Nitrate reductase and glutamine synthetase activities | |
Hong, Hua-Sheng1; Wang, Yu-Jue2,3,4 ; Wang, Da-Zhi1
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| 发表期刊 | CONTINENTAL SHELF RESEARCH
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| ISSN | 0278-4343 |
| 2011-05-01 | |
| 卷号 | 31期号:6页码:S57-S66 |
| 关键词 | The Taiwan Strait Upwelling Phytoplankton Nitrogen Addition Nitrate Reductase (Nr) Glutamine Synthetase (Gs) |
| 产权排序 | [Hong, Hua-Sheng; Wang, Da-Zhi] Xiamen Univ, State Key Lab Marine Environm Sci, Environm Sci Res Ctr, Xiamen 361005, Peoples R China; [Wang, Yu-Jue] CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Wang, Yu-Jue] Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Wang, Yu-Jue] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Wang, DZ, Xiamen Univ, State Key Lab Marine Environm Sci, Environm Sci Res Ctr, Xiamen 361005, Peoples R China.dzwang@xmu.edu.cn |
| 作者部门 | 近岸生态与环境试验室 |
| 英文摘要 | This study investigated the activities of two nitrogen assimilation enzymes, nitrate reductase (NR) and glutamine synthetase (GS) in phytoplankton in relation to sample, as well as the nutrient levels and phytoplankton biomass (Chl-a concentrations) during an upwelling event in the southern Taiwan Strait during an upwelling period from 6 to 12 July, 2005. The results showed that high NR activity (NRA) was always found with low nitrate and high Chl-a concentrations, while GS activity (GSA) exhibited positive correlations with ammonium and Chl-a concentration. Both NRA and GSA varied with the time and stage of upwelling: high NRA and GSA were observed initially at the subsurface layers in the early stage of upwelling, accompanied by the consumption of nutrients and the increase of Chl-a concentration; and then at the surface with high Chl-a concentrations in the middle and late stages of upwelling. Results from in situ enzyme bioassays on water samples along the tracing of upwelling track and on board mesocosm experiments on board the ship showed that there was a time-lag between nitrate addition and NRA and GSA, but. However, no time-lag was found between ammonium addition and GSA. The present results indicated that both NRA and GSA reflect the status of ambient nitrogen levels and the assimilation process of the phytoplankton, and could be used as effective parameters for the analysis of the physiological response of the phytoplankton to nitrogen variations during upwelling periods. Measurements of NRA and GSA in the phytoplankton in newly upwelled water appeared to provide ecophysiological indicators of phytoplankton, which will make it possible to trace during the sequence of upwelling events (such as nutrient supplementation) leading to enhanced productivity. (C) 2011 Elsevier Ltd. All rights reserved.; This study investigated the activities of two nitrogen assimilation enzymes, nitrate reductase (NR) and glutamine synthetase (GS) in phytoplankton in relation to sample, as well as the nutrient levels and phytoplankton biomass (Chl-a concentrations) during an upwelling event in the southern Taiwan Strait during an upwelling period from 6 to 12 July, 2005. The results showed that high NR activity (NRA) was always found with low nitrate and high Chl-a concentrations, while GS activity (GSA) exhibited positive correlations with ammonium and Chl-a concentration. Both NRA and GSA varied with the time and stage of upwelling: high NRA and GSA were observed initially at the subsurface layers in the early stage of upwelling, accompanied by the consumption of nutrients and the increase of Chl-a concentration; and then at the surface with high Chl-a concentrations in the middle and late stages of upwelling. Results from in situ enzyme bioassays on water samples along the tracing of upwelling track and on board mesocosm experiments on board the ship showed that there was a time-lag between nitrate addition and NRA and GSA, but. However, no time-lag was found between ammonium addition and GSA. The present results indicated that both NRA and GSA reflect the status of ambient nitrogen levels and the assimilation process of the phytoplankton, and could be used as effective parameters for the analysis of the physiological response of the phytoplankton to nitrogen variations during upwelling periods. Measurements of NRA and GSA in the phytoplankton in newly upwelled water appeared to provide ecophysiological indicators of phytoplankton, which will make it possible to trace during the sequence of upwelling events (such as nutrient supplementation) leading to enhanced productivity. (C) 2011 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | NSF [OCE-0351646]; CAS/SAFEA |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ALGA MONORAPHIDIUM-BRAUNII ; MARINE-PHYTOPLANKTON ; MONTEREY BAY ; CHLOROPHYLL ; MONSOON ; ASSAY ; PROCHLOROCOCCUS ; AVAILABILITY ; PRODUCTIVITY ; LIMITATION |
| 研究领域[WOS] | Oceanography |
| WOS记录号 | WOS:000290195700007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4863 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Xiamen Univ, State Key Lab Marine Environm Sci, Environm Sci Res Ctr, Xiamen 361005, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hong, Hua-Sheng,Wang, Yu-Jue,Wang, Da-Zhi. Response of phytoplankton to nitrogen addition in the Taiwan strait upwelling region: Nitrate reductase and glutamine synthetase activities[J]. CONTINENTAL SHELF RESEARCH,2011,31(6):S57-S66. |
| APA | Hong, Hua-Sheng,Wang, Yu-Jue,&Wang, Da-Zhi.(2011).Response of phytoplankton to nitrogen addition in the Taiwan strait upwelling region: Nitrate reductase and glutamine synthetase activities.CONTINENTAL SHELF RESEARCH,31(6),S57-S66. |
| MLA | Hong, Hua-Sheng,et al."Response of phytoplankton to nitrogen addition in the Taiwan strait upwelling region: Nitrate reductase and glutamine synthetase activities".CONTINENTAL SHELF RESEARCH 31.6(2011):S57-S66. |
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