| Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry | |
Ma, Jiping3; Xiao, Ronghui3; Li, Jinhua1,2 ; Li, Jie3; Shi, Benzhang3; Liang, Yanjuan3; Lu, Wenhui1,2,3; Chen, Lingxin1,2
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| 发表期刊 | JOURNAL OF SEPARATION SCIENCE
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| ISSN | 1615-9306 |
| 2011-06-01 | |
| 卷号 | 34期号:12页码:1477-1483 |
| 关键词 | Aldehyde Alga Derivatization Gc-ms Hs-spme |
| 产权排序 | [Li, Jinhua; Lu, Wenhui; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Li, Jinhua; Lu, Wenhui; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Ma, Jiping; Xiao, Ronghui; Li, Jie; Shi, Benzhang; Liang, Yanjuan; Lu, Wenhui] Qingdao Technol Univ, Inst Environm & Municipal Engn, Key Lab Environm Engn Shandong Prov, Qingdao, Peoples R China |
| 通讯作者 | Chen, LX, Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Chunhui Rd 17, Yantai 264003, Shandong, Peoples R China.majiping2001@yahoo.com.cn ; lxchen@yic.ac.cn |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | A simple, fast, sensitive and cost-effective method based on headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization coupled with gas chromatography-mass spectrometry was developed for the determination of six typical aldehydes, 2E-hexenal, heptanal, 2E-heptenal, 2E,4E-heptadienal, 2E-decenal and 2E,4E-decadienal in laboratory algae cultures. As derivatization reagent, O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine hydrochloride, was loaded onto the poly(dimethylsiloxane)/divinylbenzene fiber for aldehydes on-fiber derivatization prior to HS-SPME. Various influence factors of extraction efficiency were systematically investigated. Under optimized extraction conditions, excellent method performances for all the six aldehydes were attained, such as satisfactory extraction recoveries ranging from 67.1 to 117%, with the precision (relative standard deviation) within 5.3-11.1%, and low detection limits in the range of 0.026-0.044 mu g/L. The validated method was successfully applied for the analysis of the aldehydes in two diatoms (Skeletonema costatum and Chaetoceros muelleri), two pyrrophytas (Prorocentrum micans and Scrippsiella trochoidea) and Calanus sinicus eggs (feeding on the two diatoms above).; A simple, fast, sensitive and cost-effective method based on headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization coupled with gas chromatography-mass spectrometry was developed for the determination of six typical aldehydes, 2E-hexenal, heptanal, 2E-heptenal, 2E,4E-heptadienal, 2E-decenal and 2E,4E-decadienal in laboratory algae cultures. As derivatization reagent, O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine hydrochloride, was loaded onto the poly(dimethylsiloxane)/divinylbenzene fiber for aldehydes on-fiber derivatization prior to HS-SPME. Various influence factors of extraction efficiency were systematically investigated. Under optimized extraction conditions, excellent method performances for all the six aldehydes were attained, such as satisfactory extraction recoveries ranging from 67.1 to 117%, with the precision (relative standard deviation) within 5.3-11.1%, and low detection limits in the range of 0.026-0.044 mu g/L. The validated method was successfully applied for the analysis of the aldehydes in two diatoms (Skeletonema costatum and Chaetoceros muelleri), two pyrrophytas (Prorocentrum micans and Scrippsiella trochoidea) and Calanus sinicus eggs (feeding on the two diatoms above). |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences [KZCX2-YW-Q07-04]; National Science and Technology Support Program [2011BAC02B04]; CAS/SAFEA |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SINGLE-DROP MICROEXTRACTION ; ALPHA,BETA,GAMMA,DELTA-UNSATURATED ALDEHYDES ; CHEMICAL DEFENSE ; HUMAN BLOOD ; O-(2,3,4,5,6-PENTAFLUOROBENZYL)OXIME DERIVATIVES ; UNSATURATED ALDEHYDES ; COPEPOD REPRODUCTION ; DIATOMS |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000291986600015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4843 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院烟台海岸带研究所 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 3.Qingdao Technol Univ, Inst Environm & Municipal Engn, Key Lab Environm Engn Shandong Prov, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Jiping,Xiao, Ronghui,Li, Jinhua,et al. Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(12):1477-1483. |
| APA | Ma, Jiping.,Xiao, Ronghui.,Li, Jinhua.,Li, Jie.,Shi, Benzhang.,...&Chen, Lingxin.(2011).Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry.JOURNAL OF SEPARATION SCIENCE,34(12),1477-1483. |
| MLA | Ma, Jiping,et al."Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry".JOURNAL OF SEPARATION SCIENCE 34.12(2011):1477-1483. |
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