| Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum | |
Li, Chenghua1; Sun, Huili2; Chen, Aiqin1; Ning, Xuanxuan3; Wu, Huifeng1 ; Qin, Song1; Xue, Qinzhao1; Zhao, Jianmin1
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2010-03-01 | |
| 卷号 | 28期号:3页码:499-503 |
| 关键词 | Venerupis Philippinarum Iccu/zn-sod Real-time Pcr |
| 产权排序 | [Li, Chenghua; Chen, Aiqin; Wu, Huifeng; Qin, Song; Xue, Qinzhao; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Sun, Huili] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
| 通讯作者 | Zhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved; Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved |
| 文章类型 | Article |
| 资助机构 | South China Sea Institute of Oceanology, Chinese Academy of Sciences Innovation Program [kzcx2-yw-225]; NSFC [30901115] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SCALLOP ARGOPECTEN-IRRADIANS ; MESSENGER-RNA EXPRESSION ; DROSOPHILA-CATALASE GENE ; RED-BLOOD-CELLS ; MOLECULAR-CLONING ; ACATALASEMIC MUTANTS ; SIGNALING PATHWAY ; CHLAMYS-FARRERI ; CDNA CLONING ; STRESS |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000275704900013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/3755 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院烟台海岸带研究所知识产出 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chenghua,Sun, Huili,Chen, Aiqin,et al. Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(3):499-503. |
| APA | Li, Chenghua.,Sun, Huili.,Chen, Aiqin.,Ning, Xuanxuan.,Wu, Huifeng.,...&Zhao, Jianmin.(2010).Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,28(3),499-503. |
| MLA | Li, Chenghua,et al."Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 28.3(2010):499-503. |
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