Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions

doi:10.1016/j.aca.2024.342440

	Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions
	Ding, Hao 1; Zhang, Mingdi 1; Wang, Xiaochun 1; He, Shuai 2; Wang, Xiaokun 1; Chen, Lingxin3,4,5
发表期刊	ANALYTICA CHIMICA ACTA
ISSN	0003-2670
	2024-04-22
卷号	1299 页码:10
关键词	Ochratoxin A Dual-channel Signal on In-system correction Mini-program
DOI	10.1016/j.aca.2024.342440
通讯作者	Wang, Xiaokun(wxkun87@126.com)
英文摘要	Ochratoxin A (OTA) is a hazardous food contaminant with significant health risks. Dual -channel OTA detection is noted for its cross-reference capability and high accuracy. Still, challenges in addressing in -system corrections and "signal off" related false positives and limited signal gains remain. Herein, we developed a dual -channel "signal on" aptasensor with one recognition process and two independent signal outputs for OTA analysis. The OTA aptamer binds to magnetic beads (MBs) and partially hybridizes with a complementary -trigger (cDNATrigger) sequence. Adding OTA disrupts the duplex sequence, leading to G-quadruplex (G4) formation and enrichment on the MBs, which then interacts with hemin to catalyze a color signal. Concurrently, the freed cDNA-Trigger catalyzes an enzyme -free DNA circuit, producing a fluorescence signal. The magnetic enrichment and signal amplification strategies make the proposed assay demonstrate excellent sensitivity toward OTA, with limits of detection (LOD) of 0.017 pM in the fluorescence channel and 48.1 pM in the colorimetric channel. Both channels have effectively detected OTA in grape juice and baijiu, demonstrating their applicability and reliability. Moreover, given the widespread use of smartphones globally, a mini -program with a self -correction function was designed to facilitate on -site colorimetric channel monitoring, making OTA detection more accessible and user-friendly.
资助机构	National Natural Science Foundation of China
收录类别	SCI
语种	英语
关键词[WOS]	ELISA ; APTASENSOR ; ASSAY
研究领域[WOS]	Chemistry
WOS记录号	WOS:001209090700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35364
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Wang, Xiaokun
作者单位	1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 2.Univ New South Wales, Sch Mech & Mfg Engn, Sydney 2052, Australia 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 5.Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China
推荐引用方式 GB/T 7714	Ding, Hao,Zhang, Mingdi,Wang, Xiaochun,et al. Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions[J]. ANALYTICA CHIMICA ACTA,2024,1299:10.
APA	Ding, Hao,Zhang, Mingdi,Wang, Xiaochun,He, Shuai,Wang, Xiaokun,&Chen, Lingxin.(2024).Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions.ANALYTICA CHIMICA ACTA,1299,10.
MLA	Ding, Hao,et al."Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions".ANALYTICA CHIMICA ACTA 1299(2024):10.