Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates
Li, J.1; Zhang, L. L.1,2; Liu, Q. H.1,2; Xu, X. Z.1,2; Xiao, Z. Z.1; Ma, D. Y.1; Xu, S. H.1; Xue, Q. Z.3
发表期刊CRYOBIOLOGY
ISSN0011-2240
2009-08-01
卷号59期号:1页码:48-53
关键词Red Seabream Embryo Extra- And Intra-cellular Ice Formation Cooling Rate
产权排序Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol;Chinese Acad Sci, Grad Sch;Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev
通讯作者Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
作者部门污染过程与控制实验室
英文摘要The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (T-OIF), extra-cellular ice formation (T-EIF) and intracellular ice formation (T-IIF) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. T-EIF and T-IIF at high cooling rate were much lower than that at low cooling rate. And the value of T-EIF - T-IIF increased from 0.45 to 11.11 degrees C with the increase of cooling rate from 3 to 130 degrees C/min. Taken together, our results suggested that high cooling rate with proper cryoprotectant would be a good option for red seabream embryo cryopreservation. (C) 2009 Elsevier Inc. All rights reserved.
文章类型Article
资助机构National Natural Science Foundation of China [30571427]
收录类别SCI
语种英语
关键词[WOS]ZEBRAFISH DANIO-RERIO ; PARALICHTHYS-OLIVACEUS EMBRYOS ; SPARUS-AURATA EMBRYOS ; SCOPHTHALMUS-MAXIMUS ; TURBOT EMBRYOS ; HUMAN OOCYTES ; CRYOPRESERVATION ; VITRIFICATION ; SPERM ; CRYOPROTECTANTS
研究领域[WOS]Life Sciences & Biomedicine - Other Topics ; Physiology
WOS记录号WOS:000268059000008
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/3532
专题中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
中国科学院烟台海岸带研究所知识产出
作者单位1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714
Li, J.,Zhang, L. L.,Liu, Q. H.,et al. Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates[J]. CRYOBIOLOGY,2009,59(1):48-53.
APA Li, J..,Zhang, L. L..,Liu, Q. H..,Xu, X. Z..,Xiao, Z. Z..,...&Xue, Q. Z..(2009).Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates.CRYOBIOLOGY,59(1),48-53.
MLA Li, J.,et al."Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates".CRYOBIOLOGY 59.1(2009):48-53.
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