Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing

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	Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing
	Ding, Jiawang1,2,3 ; Qin, Wei1
发表期刊	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2009-10-21
卷号	131 期号:41 页码:14640-+
关键词	Uniform Concentration Profiles Current Pulse Method Detection Limits Sensors Polarization Coefficients Titrations
产权排序	Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, S China Sea Inst Oceanol;Chinese Acad Sci, Grad Univ
通讯作者	Qin, W, Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
作者部门	环境化学实验室
英文摘要	A polymeric membrane ion-selective electrode (ISE) for rapid potentiometric biosensing has been developed. Butyrylcholinesterase, as a target enzyme, is immobilized on the surface of the polymeric membrane. A macro-command-controlled procedure for switching between the potentiostatic and galvanostatic steps has been designed to provide a current-driven release of the butyrylcholine substrate for in situ biosensing of the enzyme and its inhibitors. This system has the flexibility of trapping substrate ions in the ISE inner filling solution and provides rapid, continuous, and reproducible measurements of enzymes and other bioanalytes involved in enzymatic systems.; A polymeric membrane ion-selective electrode (ISE) for rapid potentiometric biosensing has been developed. Butyrylcholinesterase, as a target enzyme, is immobilized on the surface of the polymeric membrane. A macro-command-controlled procedure for switching between the potentiostatic and galvanostatic steps has been designed to provide a current-driven release of the butyrylcholine substrate for in situ biosensing of the enzyme and its inhibitors. This system has the flexibility of trapping substrate ions in the ISE inner filling solution and provides rapid, continuous, and reproducible measurements of enzymes and other bioanalytes involved in enzymatic systems.
文章类型	Article
资助机构	Chinese Academy of Sciences [KZCX2-YW-410]; NSFC [40776058]; MOST [2007AA09Z103]; Taishan Scholar Program ; Natural Science Foundation of Shandong Province [JQ200814]
收录类别	SCI
语种	英语
关键词[WOS]	UNIFORM CONCENTRATION PROFILES ; CURRENT PULSE METHOD ; DETECTION LIMITS ; SENSORS ; POLARIZATION ; COEFFICIENTS ; TITRATIONS
研究领域[WOS]	Chemistry
WOS记录号	WOS:000271271800021
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/3431
专题	中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jiawang,Qin, Wei. Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2009,131(41):14640-+.
APA	Ding, Jiawang,&Qin, Wei.(2009).Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,131(41),14640-+.
MLA	Ding, Jiawang,et al."Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 131.41(2009):14640-+.

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