Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array

doi:10.2139/ssrn.4636337

	Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array
	Zhang, Han 1,4; Mou, Junsong 1,4; Ding, Jiawang1,2,3 ; Qin, Wei1,2,3
发表期刊	SSRN
ISSN	15565068
	2023
关键词	Antibiotics Cell death Peptides Potentiometers (electric measuring instruments)
DOI	10.2139/ssrn.4636337
英文摘要	Phenotypic antimicrobial susceptibility testing that identifies the phenotypic feature differences of bacteria after incubation with antibiotics enables reliable antibiotics screening but is restricted by bacterial proliferation rate. Here, a magneto-controlled potentiometric sensors array was developed for rapid antibiotic screening based on direct detection of apoptotic bacteria. Phosphatidylserine (PS)-binding peptide serving as bioreceptor and signal transducer was immobilized on magnetic beads (MBs-peptide), and could selectively capture apoptotic bacteria killed by antibiotics. Apoptotic bacteria binding-induced charge density change of MBs-peptide resulted in a potential change on a magneto-controlled polymeric membrane potentiometric sensor. Based on the apoptotic bacteria detection, antimicrobial activities of micrograms per milliliter antibiotics could be evaluated within 1.5 h, which were hardly achieved by current methods. In addition, the antibacterial ability of different antibiotics could be evaluated simultaneously using a potentiometric sensors array. This approach enables sensitive, general, and time-saving antibiotic screening, and may open up a new path for antibiotic susceptibility testing. © 2023, The Authors. All rights reserved.
收录类别	EI
语种	英语
EI主题词	Antibiotics ; Cell death ; Peptides ; Potentiometers (electric measuring instruments)
EI入藏号	20230418679
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34288
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
作者单位	1.CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Shandong, Yantai; 264003, China; 2.Qingdao National Laboratory for Marine Science and Technology, Shandong, Qingdao; 266237, China; 3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Shandong, Qingdao; 266071, China; 4.University of Chinese Academy of Sciences, Beijing; 100049, China
推荐引用方式 GB/T 7714	Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array[J]. SSRN,2023.
APA	Zhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2023).Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array.SSRN.
MLA	Zhang, Han,et al."Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array".SSRN (2023).