基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应

	基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应
其他题名	Response of fish diversity to hydrological connectivity of typical tidal creek system in the Yellow River Delta based on environmental DNA metabarcoding
	董志远 1,2; 陈琳琳1,2 ; 张乃鹏 4; 陈莉 1,3; 孙德斌 1,2; 倪艳梅 1,3; 李宝泉1,2
发表期刊	生物多样性
ISSN	1005-0094
	2023
卷号	31 期号:7 页码:96-110
关键词	环境DNA宏条形码鱼类多样性水文连通黄河三角洲潮沟系统
英文摘要	Aims:Hydrological connectivity is essential for maintaining biodiversity in coastal wetlands,and it is important to understand the response of fish diversity,as a major component of biodiversity,to different hydrological connectivity intensities.This study aimed to investigate the response of fish diversity to hydrological connectivity in the typical tidal creek system and verify the applicability of eDNA metabarcoding for monitoring and conserving fish diversity in the Yellow River Delta.Methods:Water samples were collected from six sites in a typical tidal creek system in the Yellow River Delta in September 2022.Fish diversity was analyzed by using eDNA metabarcoding,in which,eDNA extracted from the water samples source were amplified by PCR using the 12S rRNA classical fish primers MiFish-E and high-throughput sequencing was performed by Illumina Miseq.The OTU representative sequences were aligned to the NCBI nucleotide sequence database to obtain species taxonomic annotation information.Thirteen environmental physicochemical indicators were measured in the water column.The relationship between fish communities and environmental factors was explored based on redundancy analysis.Results:A total of 55 fish species were detected,including 27 native and 28 non-native fish species,and Perciformes dominated the fish composition.The fishes with high sequence abundance at each sample site included Acanthogobius hasta,Planiliza haematocheilu,Acanthogobius elongatus,etc.Fish diversity of the tidal creeks with different hydrological connectivity was different.Significantly higher community diversity and richness index,the highest number of species and a more even distribution of the number of individual species were found in the secondary tidal creek than in the other two tidal creeks.SiO_3~(2-)-Si,NO_3~--N,pH,salinity,NH_4~+-N,and dissolved oxygen were significantly correlated (P <0.05) with fish community structure shown by RDA analysis.Acanthogobius hasta with higher sequence abundance was positively correlated with silicate,and the sequence abundance of Planiliza haematocheilus was negatively correlated with salinity.Conclusion:This study confirmed the feasibility of using environmental DNA metabarcoding to monitor fish diversity of typical tidal water bodies in the Yellow River estuary by comparing it with previous traditional sampling data and indicated that hydrological connectivity of tidal creek systems has a significant impact on fish community structure and diversity.The results of this study are helpful to further understand the influence mechanism of coastal wetland hydrological connectivity on biological communities.
中文摘要	水文连通对于维护滨海湿地生物多样性至关重要,鱼类多样性作为生物多样性的重要组成部分,了解其对不同水文连通强度的响应具有重要意义。本研究基于环境DNA宏条形码技术(e DNA metabarcoding)检测黄河三角洲典型潮沟系统鱼类多样性,分析鱼类物种分布对不同水文连通强度潮沟生境差异的响应特征。利用12S r RNA经典鱼类引物对采集自三级潮沟系统的水样进行高通量测序,共检测出鱼类55种,其中本地鱼类27种、非本地鱼类28种,物种组成以鲈形目为主。各样点序列丰度均较高的鱼类有矛尾刺虾虎鱼(Acanthogobius hasta)、鮻(Planiliza haematocheilus)、长体刺虾虎鱼(Acanthogobius elongatus)等。鱼类多样性在不同水文连通性潮沟间差异明显,其中,二级潮沟群落多样性水平、丰富度指数、物种种类及各种鱼类类群中的个体均匀程度等都明显高于其他两级潮沟。RDA分析显示有6种环境因子与鱼类群落结构显著相关(P《0.05),分别为:硅酸盐(SiO_(3)^(2-)-Si)、硝酸盐(NO_(3)^(-)-N)、酸碱度(p H值)、盐度(SAL)、铵盐(NH_(4)^(+)–N)、溶解氧(DO)。其中,优势种矛尾刺虾虎鱼的序列丰度与硅酸盐呈正相关,鮻的序列丰度与盐度呈负相关。本研究通过与传统采样数据进行比较,证实了环境DNA宏条形码技术用于监测黄河口典型潮沟水体中鱼类多样性的可行性,表明潮沟系统水文连通性对鱼类群落结构及多样性均具有明显的影响,研究结果有助于进一步了解滨海湿地水文连通对生物群落的影响机制。
文章类型	期刊论文
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:7522125
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34212
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.中国科学院烟台海岸带研究所海岸带生物学与生物资源保护实验室,山东烟台264003; 2.中国科学院大学,北京100049; 3.烟台大学海洋学院,山东烟台264003; 4.东营市水文中心,山东东营257000
推荐引用方式 GB/T 7714	董志远,陈琳琳,张乃鹏,等. 基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应[J]. 生物多样性,2023,31(7):96-110.
APA	董志远.,陈琳琳.,张乃鹏.,陈莉.,孙德斌.,...&李宝泉.(2023).基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应.生物多样性,31(7),96-110.
MLA	董志远,et al."基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应".生物多样性 31.7(2023):96-110.