The invention relates to detection of heparins, in particular to a quick, accurate and sensitive method for detecting heparins. The method comprises the following steps of : testing potential changes caused by adding of heparins of different concentrations by using a potential tester according to specific combination of protamine in a polymer sensitive film phase and heparins in a water phase; drawing a standard work curve according to an initial potential change rate; and obtaining the concentration of heparins in an unknown sample in reference to the standard work curve. In the invention, protamine is added into a polymer film, so that the protamine is not required to be added manually; and an electrode can be directly applied to sample detection without activating, so that the method has the advantages of easiness and convenience for operating, short detection time, low operating cost, suitability for field detection, and the like.
; 陈燕
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