本发明涉及溶菌酶，具体涉及一种紫贻贝G型溶菌酶基因及其重组蛋白和应用。G型溶菌酶基因如序列表SEQ ID NO.1中的碱基序列所示。所述G型溶菌酶基因重组表达产物可制备为抗菌药物、水产品保鲜剂或水产饲料添加剂。本发明紫贻贝G型溶菌酶，包括从感染病菌的紫贻贝中提取总RNA、mRNA纯化、cDNA模板溶液制备、G型溶菌酶基因筛选、基因克隆、重组质粒构建、重组基因表达和重组蛋白纯化步骤得到该G型溶菌酶，该G型溶菌酶的氨基酸序列如序列表SEQ ID NO.2所示；该G型溶菌酶属于一类新颖的紫贻贝溶菌酶，对海洋来源的多种革兰氏阳性和阴性病原微生物均具有很强的抑制活性。
其他摘要
The invention relates to lysozyme, specifically to a Mytilus edulis G-type lysozyme gene and a recombinant protein and application thereof. The G-type lysozyme gene is represented by a base sequence of SEQ ID No. 1 in a sequence table. A recombination expression product of the G-type lysozyme gene can be used for preparing an antibacterial agent, an aquatic product antistaling agent or an aquatic product feed additive. Mytilus edulis G-type lysozyme in the invention can be prepared through the following steps : extraction of total RNA from Mytilus edulis infected with pathogens, purification of mRNA, preparation of a cDNA template solution, screening of the G-type lysozyme gene, gene cloning, construction of recombinant plasmid, expression of a recombinant gene and purification of the recombinant protein, and an amino acid sequence of the G-type lysozyme is represented by SEQ ID No. 2 in the sequence table. The G-type lysozyme belongs to a novel Mytilus edulis lysozyme and has powerful inhibitory activity on a variety of marine-derived Gram positive and negative pathogenic microorganisms.
; 吴惠丰
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