The invention belongs to the technical field of environmental pollution detection and relates to a method and a device for detection of water acute toxicity. The method realizes toxicity analysis according to a reduction degree of an ammonium ion oxidation rate after nitrite bacteria are inhibited by noxious materials. Bacteria and a selective electrode are connected in a flow analysis system in series, a sample is fed into the system and is acted by the bacteria, then a medium is transformed, an ammonium ion-containing buffer solution is used, and an ammonium ion oxidation rate of the bacteria can be obtained by calculation of change of potential signals detected by an ammonium ion selective electrode. The method utilizes a medium transition technology, eliminates matrix effects and avoids electrode pollution and damage caused by a sample. The method and the device utilize the ammonium ion selective electrode to realize ammoxidation rate determination so that interference produced by other aerobic bacteria in oxygen electrode-based dissolved oxygen determination is avoided.
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