The invention relates to the technical field of environmental and biological analysis, and particularly relates to a kit and method for detecting cobalt ions through a colorimetric method. The kit comprises a CTAB (Cetyltrimethyl Ammonium Bromide)-containing borate buffer solution, a sodium bicarbonate solution, a hydrogen peroxide solution, a potassium thiocyanate solution, a gold nanorod solution, a standard colorimetric card F and a colorimetric tube, wherein the pH of the CTAB-containing borate buffer solution is 7.4-9.6, and the concentration of the CTAB-containing borate buffer solution is 10-200 mM; the concentration of the sodium bicarbonate solution is 0.01-1 M; the concentration of the H2O2 solution is 0.01-1 M; the concentration of the potassium thiocyanate solution is 0.01-1 M. The kit and the method disclosed by the invention have the advantages of high sensitivity, good selectivity, quickness, easiness for observation by unaided eyes and the like when used for detecting the cobalt ions and can realize the detection of namely the cobalt ions in environmental and biological samples, under an optimized experiment condition.
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