The invention relates to a sensor, and particularly relates to a potential sensor for detecting dichlorotris (1, 10-phenanthroline) ruthenium (II) and a detection method and application of the potential sensor. The potential sensor comprises a working electrode, a reference electrode and a potential determinator, wherein the potential determinator is respectively connected with the working electrode and the outer reference electrode through lead wires; the working electrode and the outer reference electrode are inserted into a detection pool containing a detection solution, a polymer sensitive membrane is adhered to the bottom of the working electrode, and a power device which plays a role in solution disturbance is arranged in the detection pool. The potential sensor for detecting the dichlorotris (1, 10-phenanthroline) ruthenium (II), which is disclosed by the invention, is high in sensitivity and high in selectivity and can be used for biological sensing based on DNA. According to the potential sensor, different target molecules can be detected by selecting different nucleic acid aptamers by the biological sensing based on DNA, when in detection, the sensor can be combined with circulating isothermal chain displacement polymerization reaction, hybrid chain reaction and a nanometer material amplification technology, so that the detection sensitivity and the detection selectivity are further enhanced.
; 秦伟
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